HLA RNA Sequencing With Unique Molecular Identifiers Reveals High Allele-Specific Variability in mRNA Expression
| العنوان: | HLA RNA Sequencing With Unique Molecular Identifiers Reveals High Allele-Specific Variability in mRNA Expression |
|---|---|
| المؤلفون: | Satu Koskela, Päivi Saavalainen, Dawit A. Yohannes, Tiira Johansson, Jukka Partanen |
| المساهمون: | HUMI - Human Microbiome Research, Biosciences, Immunomics, University Management |
| المصدر: | Frontiers in Immunology, Vol 12 (2021) Frontiers in Immunology |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Graft Rejection, lcsh:Immunologic diseases. Allergy, Mrna expression, Immunology, Human leukocyte antigen, allele-specific expression, Biology, Autoimmune Diseases, 03 medical and health sciences, 0302 clinical medicine, HLA Antigens, Gene expression, Humans, Immunology and Allergy, Genetic Predisposition to Disease, RNA, Messenger, Allele, Gene, Alleles, Original Research, 030304 developmental biology, Genetics, 0303 health sciences, Messenger RNA, Sequence Analysis, RNA, Histocompatibility Testing, Histocompatibility Antigens Class I, Histocompatibility Antigens Class II, Genetic Variation, RNA, RNA sequencing, Organ Transplantation, Transplantation, HLA, Histocompatibility, 030220 oncology & carcinogenesis, 3121 General medicine, internal medicine and other clinical medicine, gene expression, unique molecular identifiers, lcsh:RC581-607 |
| الوصف: | The HLA gene complex is the most important single genetic factor in susceptibility to most diseases with autoimmune or autoinflammatory origin and in transplantation matching. Most studies have focused on the vast allelic variation in these genes; only a few studies have explored differences in the expression levels of HLA alleles. In this study, we quantified mRNA expression levels of HLA class I and II genes from peripheral blood samples of 50 healthy individuals. The gene- and allele-specific mRNA expression was assessed using unique molecular identifiers, which enabled PCR bias removal and calculation of the number of original mRNA transcripts. We identified differences in mRNA expression between different HLA genes and alleles. Our results suggest that HLA alleles are differentially expressed and these differences in expression levels are quantifiable using RNA sequencing technology. Our method provides novel insights into HLA research, and it can be applied to quantify expression differences of HLA alleles in various tissues and to evaluate the role of this type of variation in transplantation matching and susceptibility to autoimmune diseases. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6dde16724e634c6990e4b7047a802ce6 http://hdl.handle.net/10138/330669 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6dde16724e634c6990e4b7047a802ce6 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |