Microbiota Introduced to Germ-Free Rats Restores Vascular Contractility and Blood Pressure
| العنوان: | Microbiota Introduced to Germ-Free Rats Restores Vascular Contractility and Blood Pressure |
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| المؤلفون: | Xi Cheng, Cameron G. McCarthy, Saroj Chakraborty, Tao Yang, Matam Vijay-Kumar, Jiyoun Yeo, Camilla F Wenceslau, Jonnelle M Edwards, Nicole R. Bearss, Piu Saha, Bina Joe, Rachel M. Golonka, Beng San Yeoh, Blair Mell, Janara Furtado |
| المصدر: | Hypertension |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, medicine.medical_specialty, Hemodynamics, Blood Pressure, 030204 cardiovascular system & hematology, Gut flora, Article, Polymerization, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, Cell Movement, Internal medicine, Internal Medicine, medicine, Animals, Germ-Free Life, Actin, Cell Proliferation, biology, Microbiota, Cofilin, biology.organism_classification, Gastrointestinal Microbiome, Mesenteric Arteries, Specific Pathogen-Free Organisms, Actin Cytoskeleton, 030104 developmental biology, Endocrinology, Blood pressure, Phosphorylation, Hypotension, medicine.symptom, Vasoconstriction, Homeostasis |
| الوصف: | Commensal gut microbiota are strongly correlated with host hemodynamic homeostasis, but only broadly associated with cardiovascular health. This includes a general correspondence of quantitative and qualitative shifts in intestinal microbial communities found in hypertensive rat models and human patients. However, the mechanisms by which gut microbes contribute to the function of organs important for blood pressure control remain unanswered. To examine the direct effects of microbiota on blood pressure, we conventionalized germ-free (GF) rats with specific pathogen free rats for a short-term period of 10 days, which served as a model system to observe the dynamic responses when reconstituting the holobiome. The absence of microbiota in GF rats resulted with relative hypotension compared to their conventionalized counterparts, suggesting an obligatory role of microbiota in blood pressure homeostasis. Hypotension observed in GF rats was accompanied by a marked reduction in vascular contractility. Both blood pressure and vascular contractility were restored by the introduction of microbiota to GF rats, indicating that microbiota could be impacting blood pressure through a vascular-dependent mechanism. This is further supported by the decrease in actin polymerization in arteries from GF rats. Improved vascular contractility in conventionalized GF rats, as indicated through stabilized actin filaments, was associated with an increase in cofilin phosphorylation. These data indicate that the vascular system senses the presence (or lack of) microbiota to maintain vascular tone via actin polymerization. Taken together, these results constitute a fundamental discovery of the essential nature of microbiota in blood pressure regulation. |
| تدمد: | 1524-4563 0194-911X |
| DOI: | 10.1161/hypertensionaha.120.15939 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ddb7e76ff0952ebd7b0385421c5a193 https://doi.org/10.1161/hypertensionaha.120.15939 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6ddb7e76ff0952ebd7b0385421c5a193 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15244563 0194911X |
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| DOI: | 10.1161/hypertensionaha.120.15939 |