Differential expression of recently duplicated PTOX genes in Glycine max during plant development and stress conditions
| العنوان: | Differential expression of recently duplicated PTOX genes in Glycine max during plant development and stress conditions |
|---|---|
| المؤلفون: | Daniel Ferreira Feijó, José Hélio Costa, João Hermínio Martins da Silva, Rachel Alves Maia, Karine Leitão Lima Thiers, André Luiz Maia Roque, Clesivan Pereira dos Santos, Birgit Arnholdt-Schmitt, Kátia Daniella da Cruz Saraiva |
| المصدر: | Journal of Bioenergetics and Biomembranes. 51:355-370 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Plastoquinone, Physiology, Plant Development, Biology, Genes, Plant, Genome, Plastid terminal oxidase, Chloroplast Proteins, 03 medical and health sciences, 0302 clinical medicine, Gene Expression Regulation, Plant, Stress, Physiological, Gene expression, Gene duplication, Plastids, RNA, Messenger, Gene, Plant Proteins, Genetics, Phylogenetic tree, food and beverages, Cell Biology, Chlororespiration, Molecular Docking Simulation, Chloroplast, 030104 developmental biology, 030220 oncology & carcinogenesis, Soybeans, Oxidoreductases |
| الوصف: | Plastid terminal oxidase (PTOX) is a chloroplast enzyme that catalyzes oxidation of plastoquinol (PQH2) and reduction of molecular oxygen to water. Its function has been associated with carotenoid biosynthesis, chlororespiration and environmental stress responses in plants. In the majority of plant species, a single gene encodes the protein and little is known about events of PTOX gene duplication and their implication to plant metabolism. Previously, two putative PTOX (PTOX1 and 2) genes were identified in Glycine max, but the evolutionary origin and the specific function of each gene was not explored. Phylogenetic analyses revealed that this gene duplication occurred apparently during speciation involving the Glycine genus ancestor, an event absent in all other available plant leguminous genomes. Gene expression evaluated by RT-qPCR and RNA-seq data revealed that both PTOX genes are ubiquitously expressed in G. max tissues, but their mRNA levels varied during development and stress conditions. In development, PTOX1 was predominant in young tissues, while PTOX2 was more expressed in aged tissues. Under stress conditions, the PTOX transcripts varied according to stress severity, i.e., PTOX1 mRNA was prevalent under mild or moderate stresses while PTOX2 was predominant in drastic stresses. Despite the high identity between proteins (97%), molecular docking revealed that PTOX1 has higher affinity to substrate plastoquinol than PTOX2. Overall, our results indicate a functional relevance of this gene duplication in G. max metabolism, whereas PTOX1 could be associated with chloroplast effectiveness and PTOX2 to senescence and/or apoptosis. |
| تدمد: | 1573-6881 0145-479X |
| DOI: | 10.1007/s10863-019-09810-x |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d8b173937e9e4a17a92ee3a43d2bcd5 https://doi.org/10.1007/s10863-019-09810-x |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....6d8b173937e9e4a17a92ee3a43d2bcd5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15736881 0145479X |
|---|---|
| DOI: | 10.1007/s10863-019-09810-x |