A SARS-CoV-2 Peptide Spectral Library Enables Rapid, Sensitive Identification of Virus Peptides in Complex Biological Samples
| العنوان: | A SARS-CoV-2 Peptide Spectral Library Enables Rapid, Sensitive Identification of Virus Peptides in Complex Biological Samples |
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| المؤلفون: | Audrey Astori, Jonathan St-Germain, Brian Raught |
| المصدر: | Journal of Proteome Research. 20:2187-2194 |
| بيانات النشر: | American Chemical Society (ACS), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, viruses, Peptide, Computational biology, medicine.disease_cause, Proteomics, Biochemistry, Virus, 03 medical and health sciences, Peptide Library, Tandem Mass Spectrometry, Peptide spectral library, medicine, Humans, Database search engine, Peptide library, Coronavirus, chemistry.chemical_classification, 030102 biochemistry & molecular biology, SARS-CoV-2, Chemistry, fungi, HEK 293 cells, COVID-19, General Chemistry, HEK293 Cells, 030104 developmental biology, Peptides |
| الوصف: | On the basis of an analysis of (i) SARS-CoV-2 virions, (ii) SARS-CoV-2-infected VeroE6 cell lysates, and (iii) recombinant SARS-CoV-2 proteins expressed in HEK 293 cells, here we present a comprehensive SARS-CoV-2 peptide spectrum compendium, comprising 1682 high confidence peptide consensus spectra derived from 1170 peptides (of various charge states) spanning 23 virus proteins. This high quality reference set can be used, e.g., for the selection of commonly observed virus peptides for use in targeted proteomics or data-independent acquisition (DIA) approaches. Using this rich resource, we also demonstrate that a spectral matching search approach yields improved performance over the use of standard database search engines alone for the identification of virus peptides in complex biological samples. |
| تدمد: | 1535-3907 1535-3893 |
| DOI: | 10.1021/acs.jproteome.1c00048 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d81255e3bcbc375dca820fa7134f80d https://doi.org/10.1021/acs.jproteome.1c00048 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....6d81255e3bcbc375dca820fa7134f80d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15353907 15353893 |
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| DOI: | 10.1021/acs.jproteome.1c00048 |