A new method for purification ofEimeria tenella merozoites
| العنوان: | A new method for purification ofEimeria tenella merozoites |
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| المؤلفون: | Larry R. McDougald, Xie Mingquan, J. M. Gilbert, A. L. Fuller |
| المصدر: | Parasitology Research. 76:566-569 |
| بيانات النشر: | Springer Science and Business Media LLC, 1990. |
| سنة النشر: | 1990 |
| مصطلحات موضوعية: | Time Factors, animal structures, Hyaluronoglucosaminidase, Chick Embryo, Eimeria, Cecum, chemistry.chemical_compound, medicine, Animals, Parasite hosting, Trypsin, Infectivity, Taurodeoxycholic Acid, General Veterinary, biology, Embryo, General Medicine, biology.organism_classification, Infectious Diseases, medicine.anatomical_structure, Biochemistry, chemistry, Insect Science, Immunology, Parasitology, Taurodeoxycholic acid, Digestion, Chickens, medicine.drug |
| الوصف: | A rapid and simple method for purifying second generation merozoites of Eimeria tenella was developed using a host tissue digestion fluid, containing 0.25% trypsin and 0.5% taurodeoxycholic acid, to liberate merozoites grown in chick embryos or from parasitized ceca. After filtration, the digestion procedure yielded 1.4 x 10(7) or 8.33 x 10(7) merozoites per embryo or cecum, respectively. These yields were nine-fold for embryos and three-fold for ceca in comparison to previous reports. Viability of the merozoites was normal as assessed by their ability to reinfect embryos and cell cultures. The new method has advantages in that large numbers of pure, viable merozoites can be obtained quickly and easily, and the procedures require minimal effort and supplies. |
| تدمد: | 1432-1955 0044-3255 |
| DOI: | 10.1007/bf00932562 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d7cee7e60f9308326fed80adf703288 https://doi.org/10.1007/bf00932562 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....6d7cee7e60f9308326fed80adf703288 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14321955 00443255 |
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| DOI: | 10.1007/bf00932562 |