High-fat diet modifies the PPAR-γ pathway leading to disruption of microbial and physiological ecosystem in murine small intestine
| العنوان: | High-fat diet modifies the PPAR-γ pathway leading to disruption of microbial and physiological ecosystem in murine small intestine |
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| المؤلفون: | Thierry Pedron, Chek Kun Tan, Kalina Duszka, Robert Ducroc, Philippe J. Sansonetti, Céline Mulet, Rémy Burcelin, Azadeh Saffarian, Béatrice Regnault, Walter Wahli, Jean-Baptiste Cavin, Julie Tomas |
| المساهمون: | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Pathogénie microbienne moléculaire, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de recherche sur l'Inflammation (CRI (UMR_S_1149 / ERL_8252 / U1149)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Génotypage des Eucaryotes (Plate-Forme), Institut Pasteur [Paris], Nanyang Technological University [Singapour], Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Fédérale Toulouse Midi-Pyrénées, Center for Integrative Genomics - Institute of Bioinformatics, Génopode (CIG), Swiss Institute of Bioinformatics [Lausanne] (SIB), Université de Lausanne (UNIL)-Université de Lausanne (UNIL), Chaire Microbiologie et Maladies infectieuses, Collège de France (CdF (institution)), This work was supported in part by the Société Française de Nutrition, the Fondation des Treilles created by Anne Gruner Schlumberger (www.les-treilles.com), the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' (Grant ANR-10-LABX-62-IBEID), the European Research Council Advanced Grants 339579-DECRYPT (to P.J.S.), a Start-Up Grant from the Lee Kong Chian School of Medicine, Nanyang Technological University, and the 7th EU program TORNADO., We thank Laurence Motreff for her help with the Illumina libraries preparation and the MiSeq run, Eeswari Paramalingam for animal handling and tissue preparation, Jacques Ravel for editing the manuscript, and Nathalie Bechon for her contribution during her fellowship. J.T. was in part funded by the Contrat Jeune Scientifique from the Institut National de la Recherche Agronomique (Jouy-en-Josas, France)., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 339579,EC:FP7:ERC,ERC-2013-ADG,DECRYPT(2014), Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Lausanne = University of Lausanne (UNIL)-Université de Lausanne = University of Lausanne (UNIL), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP), Collège de France - Chaire Microbiologie et Maladies infectieuses, TOMAS, Julie, PEDRON, Thierry, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Decrypting signals in the crypt. - DECRYPT - - EC:FP7:ERC2014-04-01 - 2019-03-31 - 339579 - VALID, Sansonetti, Philippe J., Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Toulouse (UT), Lee Kong Chian School of Medicine (LKCMedicine) |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2016, 113 (40), pp.E5934-E5943. ⟨10.1073/pnas.1612559113⟩ Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2016, 113 (40), ⟨10.1073/pnas.1612559113⟩ Proceedings of the National Academy of Sciences of the United States of America, 2016, 113 (40), ⟨10.1073/pnas.1612559113⟩ Proceedings of the National Academy of Sciences of the United States of America, 2016, 113 (40), pp.E5934-E5943. ⟨10.1073/pnas.1612559113⟩ Proceedings of the National Academy of Sciences of the United States of America 40 (113), E5934-E5943. (2016) |
| بيانات النشر: | HAL CCSD, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Cystic Fibrosis Transmembrane Conductance Regulator, Peroxisome proliferator-activated receptor, MESH: Cecum/microbiology, Candidatus Arthromitus, MESH: Thiazolidinediones/pharmacology, Feces, antimicrobial peptides, MESH: Chlorides/metabolism, 0302 clinical medicine, Intestine, Small, [SDV.EE.ECO] Life Sciences [q-bio]/Ecology, environment/Ecosystems, MESH: Cystic Fibrosis Transmembrane Conductance Regulator/metabolism, MESH: Diet, High-Fat, MESH: Animals, CFTR, Receptor, Cecum, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, 2. Zero hunger, chemistry.chemical_classification, Multidisciplinary, biology, Microbiota, MESH: Intestine, Small/drug effects, MESH: Laser Capture Microdissection, MESH: Feces/microbiology, MESH: Gastrointestinal Microbiome*/drug effects, MESH: Intestine, Small/physiology, Cystic fibrosis transmembrane conductance regulator, 3. Good health, High-fat diet, Phenotype, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, medicine.anatomical_structure, high-fat diet, PNAS Plus, Biochemistry, Signal Transduction, medicine.medical_specialty, PPAR-γ, MESH: PPAR gamma/genetics, MESH: Rosiglitazone, Ileum, Laser Capture Microdissection, Diet, High-Fat, MESH: Phenotype, Rosiglitazone, 03 medical and health sciences, [SDV.EE.ECO]Life Sciences [q-bio]/Ecology, environment/Ecosystems, Chlorides, MESH: Mice, Inbred C57BL, Internal medicine, medicine, microbiota, Animals, MESH: Antimicrobial Cationic Peptides/metabolism, Intestinal permeability, MESH: Signal Transduction*/drug effects, [SDV.EE.IEO] Life Sciences [q-bio]/Ecology, environment/Symbiosis, medicine.disease, biology.organism_classification, Mucus, MESH: Gene Expression Regulation/drug effects, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Small intestine, MESH: Male, Gastrointestinal Microbiome, Mice, Inbred C57BL, PPAR gamma, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, MESH: PPAR gamma/metabolism, ecosytem, 030104 developmental biology, Endocrinology, Gene Expression Regulation, chemistry, MESH: Antimicrobial Cationic Peptides/genetics, biology.protein, Thiazolidinediones, MESH: Intestine, Small/microbiology, MESH: Mucus/metabolism, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, 030217 neurology & neurosurgery, Antimicrobial Cationic Peptides, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis |
| الوصف: | International audience; Diet is among the most important factors contributing to intestinal homeostasis, and basic functions performed by the small intestine need to be tightly preserved to maintain health. Little is known about the direct impact of high-fat (HF) diet on small-intestinal mucosal defenses and spatial distribution of the microbiota during the early phase of its administration. We observed that only 30 d after HF diet initiation, the intervillous zone of the ileum-which is usually described as free of bacteria-became occupied by a dense microbiota. In addition to affecting its spatial distribution, HF diet also drastically affected microbiota composition with a profile characterized by the expansion of Firmicutes (appearance of Erysipelotrichi), Proteobacteria (Desulfovibrionales) and Verrucomicrobia, and decrease of Bacteroidetes (family S24-7) and Candidatus arthromitus A decrease in antimicrobial peptide expression was predominantly observed in the ileum where bacterial density appeared highest. In addition, HF diet increased intestinal permeability and decreased cystic fibrosis transmembrane conductance regulator (Cftr) and the Na-K-2Cl cotransporter 1 (Nkcc1) gene and protein expressions, leading to a decrease in ileal secretion of chloride, likely responsible for massive alteration in mucus phenotype. This complex phenotype triggered by HF diet at the interface between the microbiota and the mucosal surface was reversed when the diet was switched back to standard composition or when mice were treated for 1 wk with rosiglitazone, a specific agonist of peroxisome proliferator-activated receptor-γ (PPAR-γ). Moreover, weaker expression of antimicrobial peptide-encoding genes and intervillous bacterial colonization were observed in Ppar-γ-deficient mice, highlighting the major role of lipids in modulation of mucosal immune defenses. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0027-8424 1091-6490 |
| DOI: | 10.1073/pnas.1612559113⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d715e572206c92d4584b1dc57bc59c5 https://hal-pasteur.archives-ouvertes.fr/pasteur-02554241 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6d715e572206c92d4584b1dc57bc59c5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00278424 10916490 |
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| DOI: | 10.1073/pnas.1612559113⟩ |