Butylparaben Is Toxic to Porcine Oocyte Maturation and Subsequent Embryonic Development Following In Vitro Fertilization
| العنوان: | Butylparaben Is Toxic to Porcine Oocyte Maturation and Subsequent Embryonic Development Following In Vitro Fertilization |
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| المؤلفون: | Tsevelmaa Nanjidsuren, Sun-Uk Kim, Hee-Chang Son, Bo-Woong Sim, Sang-Hoon Lee, Hyo-Gu Kang, Soo-Hyun Park, Pil-Soo Jeong, Deog-Bon Koo, Min Ju Kim, Bong-Seok Song |
| المصدر: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 21, Iss 3692, p 3692 (2020) Volume 21 Issue 10 |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Sus scrofa, Embryonic Development, Parabens, Fertilization in Vitro, 010501 environmental sciences, butylparaben, 01 natural sciences, Catalysis, Article, Inorganic Chemistry, Andrology, lcsh:Chemistry, 03 medical and health sciences, chemistry.chemical_compound, porcine oocyte, Human fertilization, Oogenesis, medicine, Animals, Blastocyst, Physical and Theoretical Chemistry, Molecular Biology, lcsh:QH301-705.5, Spectroscopy, 0105 earth and related environmental sciences, chemistry.chemical_classification, Butylparaben, Reactive oxygen species, Organic Chemistry, apoptosis, ROS, General Medicine, Glutathione, in vitro maturation, Oocyte, Computer Science Applications, In vitro maturation, mitochondria, 030104 developmental biology, medicine.anatomical_structure, chemistry, lcsh:Biology (General), lcsh:QD1-999, Toxicity, Oocytes, Reactive Oxygen Species |
| الوصف: | Parabens are widely used in personal care products due to their antimicrobial effects. Although the toxicity of parabens has been reported, little information is available on the toxicity of butylparaben (BP) on oocyte maturation. Therefore, we investigated the effects of various concentrations of BP (0 &mu M, 100 &mu M, 200 &mu M, 300 &mu M, 400 &mu M, and 500 &mu M) on the in vitro maturation of porcine oocytes. BP supplementation at a concentration greater than 300 &mu M significantly reduced the proportion of complete cumulus cell expansion and metaphase II oocytes compared to the control. The 300 &mu M BP significantly decreased fertilization, cleavage, and blastocyst formation rates with lower total cell numbers and a higher rate of apoptosis in blastocysts compared to the control. The BP-treated oocytes showed significantly higher reactive oxygen species (ROS) levels, and lower glutathione (GSH) levels than the control. BP significantly increased the aberrant mitochondrial distribution and decreased mitochondrial function compared to the control. BP-treated oocytes exhibited significantly higher percentage of &gamma H2AX, annexin V-positive oocytes and expression of LC3 than the control. In conclusion, we demonstrated that BP impaired oocyte maturation and subsequent embryonic development, by inducing ROS generation and reducing GSH levels. Furthermore, BP disrupted mitochondrial function and triggered DNA damage, early apoptosis, and autophagy in oocytes. |
| وصف الملف: | application/pdf |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d6bdbc75dd4129950450c4da9b592d1 https://pubmed.ncbi.nlm.nih.gov/32456265 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6d6bdbc75dd4129950450c4da9b592d1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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