Monitoring peripheral nerve degeneration in ALS by label-free stimulated Raman scattering imaging
| العنوان: | Monitoring peripheral nerve degeneration in ALS by label-free stimulated Raman scattering imaging |
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| المؤلفون: | Rosanna Zhang, Kevin Eggan, Aarti Sharma, Satomi Suzuki-Uematsu, Joanie Mok, Fake Lu, Johnny Salameh, X. Sunney Xie, Jeannie Chew, Daniel A. Mordes, Jack L. Strominger, Neil A. Shneider, Steve S.W. Han, Wenlong Yang, Jin Yuan Wang, Naoki Suzuki, Ester Leno-Duran, Feng Tian, Minbiao Ji, Yue Liu, Leonard Petrucelli |
| المصدر: | Nature Communications, Vol 7, Iss 1, Pp 1-15 (2016) Nature Communications |
| بيانات النشر: | Nature Portfolio, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Pathology, General Physics and Astronomy, Minocycline, Degeneration (medical), Electromyography, Spectrum Analysis, Raman, Mice, Superoxide Dismutase-1, 0302 clinical medicine, C9orf72, Transgenes, Amyotrophic lateral sclerosis, Myelin Sheath, Motor Neurons, Multidisciplinary, medicine.diagnostic_test, Lipids, Sciatic Nerve, Anti-Bacterial Agents, Peripheral, Disease Progression, Female, Artifacts, Algorithms, medicine.medical_specialty, Science, SOD1, Mice, Transgenic, Article, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Imaging, Three-Dimensional, medicine, Animals, Humans, Computer Simulation, Peripheral Nerves, Muscle Denervation, business.industry, Amyotrophic Lateral Sclerosis, General Chemistry, medicine.disease, 030104 developmental biology, Nerve Degeneration, Trinucleotide repeat expansion, business, 030217 neurology & neurosurgery |
| الوصف: | The study of amyotrophic lateral sclerosis (ALS) and potential interventions would be facilitated if motor axon degeneration could be more readily visualized. Here we demonstrate that stimulated Raman scattering (SRS) microscopy could be used to sensitively monitor peripheral nerve degeneration in ALS mouse models and ALS autopsy materials. Three-dimensional imaging of pre-symptomatic SOD1 mouse models and data processing by a correlation-based algorithm revealed that significant degeneration of peripheral nerves could be detected coincidentally with the earliest detectable signs of muscle denervation and preceded physiologically measurable motor function decline. We also found that peripheral degeneration was an early event in FUS as well as C9ORF72 repeat expansion models of ALS, and that serial imaging allowed long-term observation of disease progression and drug effects in living animals. Our study demonstrates that SRS imaging is a sensitive and quantitative means of measuring disease progression, greatly facilitating future studies of disease mechanisms and candidate therapeutics. Sensitive and label-free imaging methods to visualize nerve degeneration are currently lacking. Here authors show that stimulated Raman scattering (SRS) microscopy can be used to monitor peripheral nerve degeneration in mouse models of amyotrophic lateral sclerosis (ALS) and in postmortem tissue from ALS patients. |
| اللغة: | English |
| تدمد: | 2041-1723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d3ceaf283c3d7d8630de828db477271 https://doaj.org/article/60127ef2c8cc4a6295abca6013b56613 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6d3ceaf283c3d7d8630de828db477271 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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