Particulate emissions from modern and old technology wood combustion induce distinct time-dependent patterns of toxicological responses in vitro
| العنوان: | Particulate emissions from modern and old technology wood combustion induce distinct time-dependent patterns of toxicological responses in vitro |
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| المؤلفون: | Maija-Riitta Hirvonen, Thomas Brunner, Pasi Jalava, Stefanie Kasurinen, Joachim Kelz, Mikko S. Happo, Oskari Uski, Ingwald Obernberger |
| المساهمون: | Ympäristö- ja biotieteiden laitos / Toiminta |
| بيانات النشر: | Elsevier BV, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Chemokine, Cell Membrane Permeability, Time Factors, 010504 meteorology & atmospheric sciences, DNA damage, Chemokine CXCL2, Apoptosis, Inflammation, 010501 environmental sciences, Toxicology, Combustion, 01 natural sciences, Mice, Toxicity Tests, medicine, Animals, Cytotoxicity, 0105 earth and related environmental sciences, Air Pollutants, Time course, biology, Tumor Necrosis Factor-alpha, Chemistry, Macrophages, DNA, General Medicine, Wood, In vitro, Cell biology, Inflammation Cytotoxicity, RAW 264.7 Cells, 13. Climate action, Toxicity, biology.protein, Combustion emissions, Particulate Matter, medicine.symptom, Particulate matter, DNA Damage |
| الوصف: | Toxicological characterisation of combustion emissions in vitro are often conducted with macrophage cell lines, and the majority of these experiments are based on responses measured at 24 h after the exposure. The aim of this study was to investigate how significant role time course plays on toxicological endpoints that are commonly measured in vitro. The RAW264.7 macrophage cell line was exposed to PM1 samples (150 μg/ml) from biomass combustion devices representing old and modern combustion technologies for 2, 4, 8, 12, 24 and 32 h. After the exposure, cellular metabolic activity, cell membrane integrity, cellular DNA content, DNA damage and production of inflammatory markers were assessed. The present study revealed major differences in the time courses of the responses, statistical differences between the studied samples mostly limiting to differences between modern and old technology samples. Early stage responses consisted of disturbances in metabolic activity and cell membrane integrity. Middle time points revealed increases in chemokine production, whereas late-phase responses exhibited mostly increased DNA-damage, decreased membrane integrity and apoptotic activity. Altogether, these results implicate that the time point of measurement has to be considered carefully, when the toxicity of emission particles is characterised in in vitro study set-ups. final draft peerReviewed |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6cae667eec0c1eca7914ed82353fd6aa https://erepo.uef.fi/handle/123456789/4419 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6cae667eec0c1eca7914ed82353fd6aa |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |