التفاصيل البيبلوغرافية
| العنوان: |
Possible role of acylphosphatase, Bcl-2 and Fas/Fas-L system in the early changes of cardiac remodeling induced by volume overload |
| المؤلفون: |
Lidia Ibba-Manneschi, Daniele Nosi, Pietro Amedeo Modesti, Claudia Fiorillo, Chiara Nediani, A. Celli, Gianfranco Liguri, Paolo Nassi, Sandra Zecchi-Orlandini, A. M. Perna, Lucia Formigli, Vanessa Ponziani |
| المصدر: |
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1638(3):217-226 |
| بيانات النشر: |
Elsevier BV, 2003. |
| سنة النشر: |
2003 |
| مصطلحات موضوعية: |
medicine.medical_specialty, Fas Ligand Protein, Time Factors, Swine, Cardiac Volume, Sarcoplasmic reticulum, Volume overload, Apoptosis, Calcium-Transporting ATPases, Biology, Acylphosphatase, Sarcoplasmic Reticulum Calcium-Transporting ATPases, Muscle hypertrophy, Electrocardiography, Proto-Oncogene Proteins, Internal medicine, medicine, Animals, fas Receptor, Ventricular remodeling, Molecular Biology, Membrane Glycoproteins, Ventricular Remodeling, Calcium-Binding Proteins, Hemodynamics, Hypertrophy, medicine.disease, Acid Anhydride Hydrolases, Phospholamban, Endocrinology, Ca2+ pump, Molecular Medicine, Calcium, Cardiomyopathies, Acylphosphatase activity, Homeostasis, Antigens, CD95 |
| الوصف: |
To identify early adaptive processes of cardiac remodeling (CR) in response to volume overload, we investigated the molecular events that may link intracellular Ca 2+ homeostasis alterations and cardiomyocyte apoptosis. In swine heart subjected to aorto-cava shunt for 6, 12, 24, 48 and 96 h sarcoplasmic reticulum (SR) Ca 2+ pump activity was reduced until 48 h (−30%), but a recovery of control values was found at 96 h. The decrease in SR Ca 2+ -ATPase (SERCA2a) expression at 48 h, was more marked (−60%) and not relieved by a subsequent recovery, while phospholamban (PLB) concentration and phosphorylation were unchanged at all the considered times. Conversely, acylphosphatase activity and expression significantly increased from 48 to 96 h (+40%). Bcl-2 expression increased significantly from 6 to 24 h, but at 48 h, returned to control values. At 48 h, microscopic observations showed that overloaded myocardium underwent substantial damage and apoptotic cell death in concomitance with an enhanced Fas/Fas-L expression. At 96 h, apoptosis appeared attenuated, while Fas/Fas-L expression was still higher than control values and cardiomyocyte hypertrophy became to develop. These data suggest that in our experimental model, acylphosphatase could be involved in the recovery of SERCA2a activity, while cardiomyocyte apoptosis might be triggered by a decline in Bcl-2 expression and a concomitant activation of Fas. |
| تدمد: |
0925-4439 |
| DOI: |
10.1016/s0925-4439(03)00086-3 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c78a1a0af70591b9d179d9ad783c910 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....6c78a1a0af70591b9d179d9ad783c910 |
| قاعدة البيانات: |
OpenAIRE |