A biophotonic approach to measure pH in small volumes in vitro: Quantifiable differences in metabolic flux around the cumulus‐oocyte‐complex (COC)
| العنوان: | A biophotonic approach to measure pH in small volumes in vitro: Quantifiable differences in metabolic flux around the cumulus‐oocyte‐complex (COC) |
|---|---|
| المؤلفون: | Malcolm S. Purdey, Andrew D. Abell, Kylie R. Dunning, Erik P. Schartner, Jeremy G. Thompson, Hanna J. McLennan, Avishkar Saini, Tanya M. Monro, Georgina M. Sylvia |
| المساهمون: | McLennan, Hanna J, Saini, Avishkar, Sylvia, Georgina M, Schartner, Erik P, Dunning, Kylie R, Purdey, Malcolm S, Monro, Tanya M, Abell, Andrew D, Thompson, Jeremy G |
| المصدر: | Journal of Biophotonics. 13 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Technology, DNA damage, General Physics and Astronomy, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, 010309 optics, Mice, chemistry.chemical_compound, fluorescence sensing, 0103 physical sciences, medicine, Extracellular, Animals, General Materials Science, Lactic Acid, Chemistry, 010401 analytical chemistry, cumulus oocyte complex, lactic acid, pH sensing, General Engineering, General Chemistry, Hydrogen-Ion Concentration, Oocyte, Photobleaching, In vitro, biophotonic, 0104 chemical sciences, Staining, Lactic acid, medicine.anatomical_structure, op-tical fibre, Oocytes, Biophysics, biomarker, Follicle Stimulating Hormone, Phototoxicity, micro-environment |
| الوصف: | Unfertilised eggs (oocytes) release chemical biomarkers into the medium surrounding them. This provides an opportunity to monitor cell health and development during assisted reproductive processes if detected in a non-invasive manner. Here we report the measurement of pH using an optical fibre probe, OFP1, in 5 μL drops of culture medium containing single mouse cumulus oocyte complexes (COCs). This allowed for the detection of statistically significant differences in pH between COCs in culture medium with no additives and those incubated with either a chemical (cobalt chloride) or hormonal treatment (follicle stimulating hormone); both of which serve to induce the release of lactic acid into the medium immediately surrounding the COC. Importantly, OFP1 was shown to be cell-safe with no inherent cell toxicity or light-induced phototoxicity indicated by negative DNA damage staining. Pre-measurement photobleaching of the probe reduced fluorescence signal variability, providing improved measurement precision (0.01-0.05 pH units) compared to previous studies. This optical technology presents a promising platform for the measurement of pH and the detection of other extracellular biomarkers to assess cell health during assisted reproduction. Refereed/Peer-reviewed |
| تدمد: | 1864-0648 1864-063X |
| DOI: | 10.1002/jbio.201960038 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c370983f857edef36323eb4ffef0e3f https://doi.org/10.1002/jbio.201960038 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6c370983f857edef36323eb4ffef0e3f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18640648 1864063X |
|---|---|
| DOI: | 10.1002/jbio.201960038 |