Strongylopus grayii tadpole blastema extract exerts cytotoxic effects on embryonal rhabdomyosarcoma cells
| العنوان: | Strongylopus grayii tadpole blastema extract exerts cytotoxic effects on embryonal rhabdomyosarcoma cells |
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| المؤلفون: | Vincent Harrison, Saif F. Khan, Victoria Damerell, Jenna Bleloch, KN. ArulJothi, Musalula Sinkala, Katie Lennard, Nicola Mulder, Bridget Calder, Jonathan Blackburn, Sharon Prince |
| المصدر: | In Vitro Cellular & Developmental Biology - Animal. 58:679-692 |
| بيانات النشر: | Springer Science and Business Media LLC, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Cell Line, Tumor, Larva, Carcinogens, Animals, Antineoplastic Agents, Rhabdomyosarcoma, Embryonal, Cyclin A, Cell Biology, General Medicine, Cyclin B1, Poly(ADP-ribose) Polymerase Inhibitors, Cyclin-Dependent Kinase Inhibitor p16, Developmental Biology |
| الوصف: | Amphibians have regenerative capacity and are resistant to developing cancer. This suggests that the developing blastema, located at the tissue regeneration site, may secrete anti-cancer factors. Here, we investigate the anti-cancer potential of tadpole tail blastema extracts (TAD) from the stream frog, Strongylopus grayii, in embryonal rhabdomyosarcoma (ERMS) cells. ERMS originates in skeletal muscle tissue and is a common pediatric soft tissue sarcoma. We show using MTT assays that TAD inhibited ERMS cell viability in a concentration-dependent manner, and phase contrast/fluorescent microscopy revealed that it induced morphological markers of senescence and apoptosis. Western blotting showed that this was associated with DNA damage (γH2AX) and activation of the p38/MAPK stress signaling pathway as well as molecular markers of senescence (p16 |
| تدمد: | 1543-706X 1071-2690 |
| DOI: | 10.1007/s11626-022-00714-6 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b2a023db46ef49cc669cb63d10f4191 https://doi.org/10.1007/s11626-022-00714-6 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....6b2a023db46ef49cc669cb63d10f4191 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1543706X 10712690 |
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| DOI: | 10.1007/s11626-022-00714-6 |