In vitro-induced resistance to the deoxycytidine analogues cytarabine (AraC) and 5-aza-2′-deoxycytidine (DAC) in a rat model for acute myeloid leukemia is mediated by mutations in the deoxycytidine kinase (dck) gene
| العنوان: | In vitro-induced resistance to the deoxycytidine analogues cytarabine (AraC) and 5-aza-2′-deoxycytidine (DAC) in a rat model for acute myeloid leukemia is mediated by mutations in the deoxycytidine kinase (dck) gene |
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| المؤلفون: | R. Willemze, M. W. Honders, A. P. A. Stegmann, J. E. Landegent, A. Hagemeijer, B. Hoebee |
| المصدر: | Annals of Hematology. 71:41-47 |
| بيانات النشر: | Springer Science and Business Media LLC, 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | Molecular Sequence Data, Drug Resistance, Decitabine, Antineoplastic Agents, Chromosomal translocation, Biology, Translocation, Genetic, chemistry.chemical_compound, Deoxycytidine Kinase, Tumor Cells, Cultured, medicine, Animals, Point Mutation, Metaphase, In Situ Hybridization, Fluorescence, Polymorphism, Single-Stranded Conformational, Chromosome Aberrations, Base Sequence, medicine.diagnostic_test, Cytarabine, Cytidine, Hematology, General Medicine, Deoxycytidine kinase, Molecular biology, Rats, Leukemia, Myeloid, Acute, chemistry, Azacitidine, Deoxycytidine, medicine.drug, Fluorescence in situ hybridization |
| الوصف: | The deoxycytidine kinase (dck) gene encodes the enzyme responsible for the metabolic activation of the antileukemic drugs cytosine arabinoside (AraC) and 5-aza-2'-deoxycytidine (decitabine, DAC). The dck locus was analyzed at the chromosomal and the molecular level in a model of rat leukemic cell lines, in which AraC and DAC resistance was induced, that was marked by dck deficiency. At the chromosomal level, karyotype analysis of metaphase spreads revealed the presence of an aberrant 2q + chromosome in the AraC-resistant cell line and a (Xq:11q) translocation in its subclone RA/7. The DAC-resistant lines were identical to the parental RCL/O. Fluorescence in situ hybridization on normal rat fibroblast metaphase spreads localized the rat dck gene to chromosome 14q21-q22, a region that was not involved in any of the observed karyotypic aberrations. Analysis at the molecular level revealed an identical rearrangement of the dck gene in the AraC-resistant cell line RCL/A and its subclone RA/7 that resulted in the absence of dck expression, as assessed by RT-PCR. No genomic rearrangements were observed in a DAC-resistant cell line RCL/D or in its subclone RD/1. However, detection of a single-stranded conformation polymorphism (SSCP) allowed the identification of a single C to G substitution (His to Gln) in the dck cDNA of the DAC-resistant RD/1 clone. The data demonstrate that exposure to AraC and DAC induces a resistant phenotype marked by functional dck deficiency that may be the consequence of mutations occurring in the dck gene. |
| تدمد: | 1432-0584 0939-5555 |
| DOI: | 10.1007/bf01696231 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b1fd927693f4b0f08140fe9653638c0 https://doi.org/10.1007/bf01696231 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....6b1fd927693f4b0f08140fe9653638c0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320584 09395555 |
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| DOI: | 10.1007/bf01696231 |