Glutamine Synthetase of Streptomyces cattleya: Purification and Regulation of Synthesis
| العنوان: | Glutamine Synthetase of Streptomyces cattleya: Purification and Regulation of Synthesis |
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| المؤلفون: | Stanley L. Streicher, Philip S. Paress |
| المصدر: | Microbiology. 131:1903-1910 |
| بيانات النشر: | Microbiology Society, 1985. |
| سنة النشر: | 1985 |
| مصطلحات موضوعية: | Growth medium, Methionine, Streptomyces cattleya, Nitrogen, Glutamine, Biology, medicine.disease_cause, Microbiology, Chromatography, Affinity, Streptomyces, Cattleya, chemistry.chemical_compound, chemistry, Biochemistry, Biosynthesis, Glutamate-Ammonia Ligase, Glutamine synthetase, Spore germination, medicine, Electrophoresis, Polyacrylamide Gel |
| الوصف: | Summary: Glutamine synthetase (GS; EC 6.3.1.2) from Streptomyces cattleya was purified using a single affinity-gel chromatography step, and some of its properties were determined. Levels of GS in S. cattleya cells varied by a factor of 8 depending upon the source of nitrogen in the growth medium. Of 24 nitrogen sources examined only glutamine or NH4Cl utilization resulted in very low GS activity. Addition of NH4Cl to a culture with high GS levels appeared to stop further synthesis and resulted in a progressive decrease in the specific activity of the enzyme. The GS inhibitor methionine sulphoximine (MSX) inhibited GS activity but had no effect on exponentially growing cells. The presence of MSX either lengthened or shortened the period between spore inoculation and initiation of exponential growth, depending on the source of nitrogen. In glutamine minimal medium MSX produced earlier and more efficient spore germination while in glutamate or nitrate minimal medium germination was delayed by its presence. |
| تدمد: | 1465-2080 1350-0872 |
| DOI: | 10.1099/00221287-131-8-1903 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6abfac974ab55796d1c44343de3727f5 https://doi.org/10.1099/00221287-131-8-1903 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6abfac974ab55796d1c44343de3727f5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14652080 13500872 |
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| DOI: | 10.1099/00221287-131-8-1903 |