Application of HB17, an Arabidopsis class II homeodomain-leucine zipper transcription factor, to regulate chloroplast number and photosynthetic capacity
| العنوان: | Application of HB17, an Arabidopsis class II homeodomain-leucine zipper transcription factor, to regulate chloroplast number and photosynthetic capacity |
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| المؤلفون: | Oliver J. Ratcliffe, Shiv B. Tiwari, Paul J. Loida, T. Lynne Reuber, J. Philip Taylor, Elena A. Rice, Peter P. Repetti, Robert A. Creelman, Elizabeth A. Kohl, Melissa C. Hong, Graham J. Hymus, Don R. Maszle, Suqin Cai, Namitha Channa, Hans E. Holtan, Colleen M. Marion, Marjorie R. Lundgren, Rebecca L. Thompson |
| المصدر: | Journal of Experimental Botany |
| بيانات النشر: | Oxford University Press (OUP), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Leucine zipper, Chloroplasts, Light, Physiology, Photoperiod, Arabidopsis, Plant Science, Genes, Plant, Plant Roots, transcription factor, Gene Expression Regulation, Plant, RNA, Messenger, Photosynthesis, Gene, Transcription factor, In Situ Hybridization, photosynthetic capacity, Homeodomain Proteins, Genetics, Leucine Zippers, biology, Arabidopsis Proteins, Chloroplast number, Meristem, Plants, Genetically Modified, biology.organism_classification, Photosynthetic capacity, Cell biology, Chloroplast, Phenotype, homeodomain-leucine zipper, Mutation, chloroplast size, Mesophyll Cells, Functional genomics, leaf development, Research Paper, Transcription Factors |
| الوصف: | Transcription factors are proposed as suitable targets for the control of traits such as yield or food quality in plants. This study reports the results of a functional genomics research effort that identified ATHB17, a transcription factor from the homeodomain-leucine zipper class II family, as a novel target for the enhancement of photosynthetic capacity. It was shown that ATHB17 is expressed natively in the root quiescent centre (QC) from Arabidopsis embryos and seedlings. Analysis of the functional composition of genes differentially expressed in the QC from a knockout mutant (athb17-1) compared with its wild-type sibling revealed the over-representation of genes involved in auxin stimulus, embryo development, axis polarity specification, and plastid-related processes. While no other phenotypes were observed in athb17-1 plants, overexpression of ATHB17 produced a number of phenotypes in Arabidopsis including enhanced chlorophyll content. Image analysis of isolated mesophyll cells of 35S::ATHB17 lines revealed an increase in the number of chloroplasts per unit cell size, which is probably due to an increase in the number of proplastids per meristematic cell. Leaf physiological measurements provided evidence of improved photosynthetic capacity in 35S::ATHB17 lines on a per unit leaf area basis. Estimates of the capacity for ribulose-1,5-bisphosphate-saturated and -limited photosynthesis were significantly higher in 35S::ATHB17 lines. |
| تدمد: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/ert261 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a856311d6c485ead4782101572430e0 https://doi.org/10.1093/jxb/ert261 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6a856311d6c485ead4782101572430e0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602431 00220957 |
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| DOI: | 10.1093/jxb/ert261 |