Genome duplication in Leishmania major relies on persistent subtelomeric DNA replication
| العنوان: | Genome duplication in Leishmania major relies on persistent subtelomeric DNA replication |
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| المؤلفون: | Jeziel D. Damasceno, Catarina A. Marques, Kathryn Crouch, Richard McCulloch, Dario Beraldi, Luiz R. O. Tosi, Ricardo Obonaga, Craig Lapsley |
| المصدر: | eLife, Vol 9 (2020) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP eLife |
| بيانات النشر: | eLife Sciences Publications, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Cell division, DNA replication initiation, QH301-705.5, Science, Base J, Genomics, DNA replication, Biology, Genome, Chromosomes, General Biochemistry, Genetics and Molecular Biology, S Phase, Histones, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Gene Duplication, Gene duplication, origin, Biology (General), Leishmania major, 030304 developmental biology, Leishmania, Genetics, Microbiology and Infectious Disease, 0303 health sciences, General Immunology and Microbiology, General Neuroscience, Genetics and Genomics, General Medicine, Subtelomere, Chromosome Structures, chemistry, subtelomere, Medicine, Other, METABOLISMO, Genome, Protozoan, 030217 neurology & neurosurgery, Research Article |
| الوصف: | DNA replication is needed to duplicate a cell’s genome in S phase and segregate it during cell division. Previous work inLeishmaniadetected DNA replication initiation at just a single region in each chromosome, an organisation predicted to be insufficient for complete genome duplication within S phase. Here, we show that acetylated histone H3 (AcH3), base J and a kinetochore factor co-localise in each chromosome at only a single locus, which corresponds with previously mapped DNA replication initiation regions and is demarcated by localised G/T skew and G4 patterns. In addition, we describe previously undetected subtelomeric DNA replication in G2/M and G1-phase-enriched cells. Finally, we show that subtelomeric DNA replication, unlike chromosome-internal DNA replication, is sensitive to hydroxyurea and dependent on 9-1-1 activity. These findings indicate thatLeishmania’s genome duplication programme employs subtelomeric DNA replication initiation, possibly extending beyond S phase, to support predominantly chromosome-internal DNA replication initiation within S phase. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2050-084X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a7e04b200a403e79721476e726095db https://eprints.gla.ac.uk/223085/1/223085.pdf |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6a7e04b200a403e79721476e726095db |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2050084X |
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