Early remodeling of rat cardiac muscle induced by swimming training
| العنوان: | Early remodeling of rat cardiac muscle induced by swimming training |
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| المؤلفون: | Oscar H. P. Ramos, Sérgio Eduardo de Andrade Perez, Anselmo Sigari Moriscot, R. A. Mesquita, R. M. M. Verzola, Heloisa S. Selistre-de-Araujo, Sabrina Messa Peviani |
| المصدر: | Brazilian Journal of Medical and Biological Research, Vol 39, Iss 5, Pp 621-627 (2006) Brazilian Journal of Medical and Biological Research v.39 n.5 2006 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Volume: 39, Issue: 5, Pages: 621-627, Published: MAY 2006 ResearcherID |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Male, Time Factors, Physiology, Myosin heavy chain expression, Biochemistry, Polymerase Chain Reaction, Muscle hypertrophy, Gene expression, Myosin, General Pharmacology, Toxicology and Pharmaceutics, lcsh:QH301-705.5, lcsh:R5-920, Ventricular Remodeling, Chemistry, General Neuroscience, Physiological condition, Cardiac muscle, General Medicine, Anatomy, Organ Size, medicine.anatomical_structure, Real-time polymerase chain reaction, lcsh:Medicine (General), Anaerobic exercise, medicine.medical_specialty, Immunology, Blotting, Western, Biophysics, Rat myocardium, Internal medicine, Physical Conditioning, Animal, medicine, Training, Animals, Zymography, Lactic Acid, RNA, Messenger, Rats, Wistar, Swimming, Myosin Heavy Chains, Myocardium, Body Weight, Cell Biology, Matrix Metalloproteinases, Rats, Real time PCR, Endocrinology, lcsh:Biology (General), Gene Expression Regulation |
| الوصف: | The aim of the present investigation was to study the effect of acute swimming training with an anaerobic component on matrix metallopeptidase (MMP) activity and myosin heavy chain gene expression in the rat myocardium. Animals (male Wistar rats, weighing approximately 180 g) were trained for 6 h/day in 3 sessions of 2 h each for 1 to 5 consecutive days (N = 5 rats per group). Rats swam in basins 47 cm in diameter and 60 cm deep filled with water at 33 to 35 degrees C. After the training period a significant increase (P0.05) was observed in the heart weight normalized to body weight by about 22 and 35% in the groups that trained for 96 and 120 h, respectively. Blood lactate levels were significantly increased (P0.05) in all groups after all training sessions, confirming an anaerobic component. However, lactate levels decreased (P0.05) with days of training, suggesting that the animals became adapted to this protocol. Myosin heavy chain-beta gene expression, analyzed by real time PCR and normalized with GAPDH gene expression, showed a significant two-fold increase (P0.01) after 5 days of training. Zymography analysis of myocardium extracts indicated a single approximately 60-kDa activity band that was significantly increased (P0.05) after 72, 96, and 120 h, indicating an increased expression of MMP-2 and suggesting precocious remodeling. Furthermore, the presence of MMP-2 was confirmed by Western blot analysis, but not the presence of MMP-1 and MMP-3. Taken together, our results indicate that in these training conditions, the rat heart undergoes early biochemical and functional changes required for the adaptation to the new physiological condition by tissue remodeling. |
| وصف الملف: | text/html |
| تدمد: | 0100-879X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a752bdfa55d9193e81c24fbcb7cc7dc https://pubmed.ncbi.nlm.nih.gov/16648900 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6a752bdfa55d9193e81c24fbcb7cc7dc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 0100879X |
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