التفاصيل البيبلوغرافية
| العنوان: |
Additional file 1: of Gene correction of HBB mutations in CD34+ hematopoietic stem cells using Cas9 mRNA and ssODN donors |
| المؤلفون: |
Antony, Justin, Ngadhnjim Latifi, A. Haque, Lamsfus-Calle, Andrés, Daniel-Moreno, Alberto, Graeter, Sebastian, Baskaran, Praveen, Weinmann, Petra, Mezger, Markus, Handgretinger, Rupert, Kormann, Michael |
| بيانات النشر: |
figshare, 2018. |
| سنة النشر: |
2018 |
| الوصف: |
Figure S1. Strategy for targeting the promoter and IVS1–110 mutation of the HBB gene. A) The promoter region of HBB gene targeted with three different gene-editing tools, HBBIVS1–110 targeted with CRISPR/Cas9. B) The design of three different gene-editing tools at sequence level. Figure S2 Comparison of three different gene-editing tools at HBB promoter. The complete raw data of Fig. 1a. Figure S3 Gene correction of HBBIVS1–110 in CD34+ HSCs using pX330.sg HBBIVS1–110 and ssODNs. A) CD34+ HSCs nucleofected with pX330.sg HBBIVS1–110 plasmid and ssODNs and measured for indel rate by T7 assay and HDR by TIDE analysis. Only 5TS resulted 3% HDR rate in TIDE analysis (as in Fig. 2a). B) Gene-editing capacity of pDNA-encoded Cas9 and mRNA-encoded Cas9 were compared, and superiority of Cas9 mRNA was observed (P |
| DOI: |
10.6084/m9.figshare.7345352.v1 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a3ddd8fff5bee692b0b30af030c2cf3 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....6a3ddd8fff5bee692b0b30af030c2cf3 |
| قاعدة البيانات: |
OpenAIRE |