Characterization of the Genome of the Dairy Lactobacillus helveticus Bacteriophage ΦAQ113
| العنوان: | Characterization of the Genome of the Dairy Lactobacillus helveticus Bacteriophage ΦAQ113 |
|---|---|
| المؤلفون: | Angelarita Armiento, Anna Moles, Armando Felsani, Roberto Ramoni, Rita Grandori, Alessandro Guffanti, Miriam Zago, Jean-Baptiste Claude, Stefano Grolli, Lia Rossetti, Erika Scaltriti, Paolo Pavan, Domenico Carminati, Elena Brini, Maria Emanuela Fornasari, Giorgio Giraffa, Annalisa D'Urzo |
| المساهمون: | Zago, M, Scaltriti, E, Rossetti, L, Guffanti, A, Armiento, A, Fornasari, M, Grolli, S, Carminati, D, Brini, E, Pavan, P, Felsani, A, D'Urzo, A, Moles, A, Claude, J, Grandori, R, Ramoni, R, Giraffa, G |
| المصدر: | Applied and Environmental Microbiology. 79:4712-4718 |
| بيانات النشر: | American Society for Microbiology, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Spectrometry, Mass, Electrospray Ionization, Phage display, viruses, Molecular Sequence Data, Sequence Homology, Genetics and Molecular Biology, Genome, Viral, Biology, Lactobacillus gasseri, Polymerase Chain Reaction, Applied Microbiology and Biotechnology, Genome, Microbiology, Bacteriophage, Open Reading Frames, Lysogenic cycle, Phylogeny, Lactobacillus johnsonii, Genetics, Base Composition, Lactobacillus helveticus, Ecology, food industry, proteomics, complete genome sequence, structural proteins, mass spectrometry, food and beverages, Sequence Analysis, DNA, biology.organism_classification, Temperateness, Myoviridae, DNA, Viral, Food Science, Biotechnology |
| الوصف: | The complete genomic sequence of the dairy Lactobacillus helveticus bacteriophage ΦAQ113 was determined. Phage ΦAQ113 is a Myoviridae bacteriophage with an isometric capsid and a contractile tail. The final assembled consensus sequence revealed a linear, circularly permuted, double-stranded DNA genome with a size of 36,566 bp and a G+C content of 37%. Fifty-six open reading frames (ORFs) were predicted, and a putative function was assigned to approximately 90% of them. The ΦAQ113 genome shows functionally related genes clustered together in a genome structure composed of modules for DNA replication/regulation, DNA packaging, head and tail morphogenesis, cell lysis, and lysogeny. The identification of genes involved in the establishment of lysogeny indicates that it may have originated as a temperate phage, even if it was isolated from natural cheese whey starters as a virulent phage, because it is able to propagate in a sensitive host strain. Additionally, we discovered that the ΦAQ113 phage genome is closely related to Lactobacillus gasseri phage KC5a and Lactobacillus johnsonii phage Lj771 genomes. The phylogenetic similarities between L. helveticus phage ΦAQ113 and two phages that belong to gut species confirm a possible common ancestral origin and support the increasing consideration of L. helveticus as a health-promoting organism. |
| تدمد: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.00620-13 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6981368a489509c1902f8d289438701a https://doi.org/10.1128/aem.00620-13 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6981368a489509c1902f8d289438701a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985336 00992240 |
|---|---|
| DOI: | 10.1128/aem.00620-13 |