A new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, Habrobracon hebetor (Hymenoptera: Braconidae): Promising molecular tools for studying the population genetics of several beneficial braconid species
| العنوان: | A new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, Habrobracon hebetor (Hymenoptera: Braconidae): Promising molecular tools for studying the population genetics of several beneficial braconid species |
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| المؤلفون: | Laure Benoit, Nathalie Gauthier, Madougou Garba, Anne Loiseau |
| المساهمون: | Direct Gen Protect Vegetaux, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), West Africa Agricultural Productivity Program (WAAP/PPAAO) in Niger and the Institut de Recherche pour le Développement (IRD), France through a collaborative programme between 'La Direction Générale de Protection des Végétaux', Niamey and the IRD., Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Direction Générale de la Protection des Végétaux |
| المصدر: | European Journal of Entomology European Journal of Entomology, Czech Entomological Society, 2016, 113, pp.265-269. ⟨10.14411/eje.2016.031⟩ European Journal of Entomology, Vol 113, Iss 1, Pp 265-269 (2016) European Journal of Entomology, 2016, 113, pp.265-269. ⟨10.14411/eje.2016.031⟩ |
| بيانات النشر: | HAL CCSD, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Linkage disequilibrium, microsatellite, [SDV]Life Sciences [q-bio], Population, Population genetics, Habrobracon hebetor, biological control, Locus (genetics), Biology, parasitic wasp, 01 natural sciences, Gene flow, Loss of heterozygosity, 03 medical and health sciences, Braconidae, education, Genetics, Genetic diversity, education.field_of_study, Bracon spp, population genetics, H10 - Ravageurs des plantes, Hymenoptera, J11 - Manutention, transport, stockage et conservation des produits d'origine végétale, 010602 entomology, 030104 developmental biology, QL1-991, Insect Science, Microsatellite, gene flow, Zoology |
| الوصف: | Combining a biotin-enrichment protocol and 454GS-FLX titanium pyrosequencing technology, we characterised 22 polymorphic microsatellite loci from the parasitic wasp, Habrobracon hebetor (Say) (Hymenoptera: Braconidae), a cosmopolitan species commonly used in biological control against a wide range of both major lepidopterous pests of stored products and field crops in different parts of the world. Three multiplex PCR sets were optimised and characterised across 46 H. hebetor specimens from two samples collected from millet fields in Niger. Two to 11 alleles were found per locus and observed heterozygosity ranged from 0.289 to 0.826. Polymorphism was detected in both samples with a similar level of observed heterozygosity (0.482 vs. 0.502) and number of alleles (4.1 vs. 3.6). Deviation from Hardy-Weinberg equilibrium was detected at the same five loci in both samples and five or seven more loci in each sample but was not associated with heterozygote deficiencies. Even though evidence for linkage disequilibrium was found between a few alleles, these new loci segregated independently. The variability of the 22 loci will enable estimates of genetic diversity and structure patterns, as well as gene flow between H. hebetor populations at different spatial scales. Cross-species amplifications were successful among the six Bracon spp. tested and nine loci will be particularly appropriate for population genetic studies in B. brevicornis. |
| وصف الملف: | text/pdf; text |
| اللغة: | English |
| تدمد: | 1210-5759 1802-8829 |
| DOI: | 10.14411/eje.2016.031⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::690cfd8d720807df96b06272ecaa5472 https://hal.archives-ouvertes.fr/hal-01602396 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....690cfd8d720807df96b06272ecaa5472 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 12105759 18028829 |
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| DOI: | 10.14411/eje.2016.031⟩ |