A phosphorylated zinc finger peptide bearing a gadolinium complex for zinc detection by MRI
| العنوان: | A phosphorylated zinc finger peptide bearing a gadolinium complex for zinc detection by MRI |
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| المؤلفون: | Kyangwi P. Malikidogo, Agnès Pallier, Frédéric Szeremeta, Célia S. Bonnet, Olivier Sénèque |
| المساهمون: | Physiochimie des Métaux (PMB), Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Dalton Transactions Dalton Transactions, 2023, 52 (19), pp.6260-6266. ⟨10.1039/d3dt00728f⟩ |
| بيانات النشر: | Royal Society of Chemistry (RSC), 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Inorganic Chemistry, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, [CHIM.COOR]Chemical Sciences/Coordination chemistry |
| الوصف: | International audience; Two zinc finger peptides, namely ZFQD(Ln) and ZFQE(Ln) (Ln = Tb or Gd), with an appended Ln(3+) chelate and a phosphoserine able to coordinate the Ln(3+) ion are presented. The two peptides differ by the amino acid anchorage of the chelate, either aspartate (D) or glutamate (E). Both peptides are able to bind Zn2+ and adopt the beta beta alpha fold. Interestingly, ZFQE(Tb) shows a decrease in sensitized Tb3+ luminescence upon Zn2+ binding whereas ZFQD(Tb) does not. The luminescence change upon Zn2+ binding is attributed to a change in hydration number (q) of the Tb3+ ion due to the decoordination of the phosphoserine from the Ln(3+) ion upon Zn2+ binding and peptide folding. This process is highly sensitive to the length of the linker between the Ln chelate and the peptidic backbone. The magnetic properties of the gadolinium analogue ZFQE(Gd) were studied. An impressive relaxivity increase of 140% is observed at 60 MHz and 25 degrees C upon Zn2+ binding. These changes can be attributed to a combined increase effect of the hydration number of Gd3+ and of the rigidity of the system upon Zn2+ binding. Phantom MR images at 9.4 T show a clear signal enhancement in the presence of Zn2+. These zinc finger peptides offer a unique platform to design such Zn-responsive probes. |
| تدمد: | 1477-9234 1477-9226 |
| DOI: | 10.1039/d3dt00728f |
| DOI: | 10.1039/d3dt00728f⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68f5a222ca6d77031adc32f54b92063c https://doi.org/10.1039/d3dt00728f |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....68f5a222ca6d77031adc32f54b92063c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779234 14779226 |
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| DOI: | 10.1039/d3dt00728f |