Sex Hormones Promote Opposite Effects on ACE and ACE2 Activity, Hypertrophy and Cardiac Contractility in Spontaneously Hypertensive Rats
| العنوان: | Sex Hormones Promote Opposite Effects on ACE and ACE2 Activity, Hypertrophy and Cardiac Contractility in Spontaneously Hypertensive Rats |
|---|---|
| المؤلفون: | Rogério Faustino Ribeiro, Sonia Alves Gouvea, Gláucia R. Abreu, Margareth Ribeiro Moysés, A.Z. Lamas, Marcio Fernando Madureira Alves, Tadeu Uggere de Andrade, Adriana K. Carmona, Nazaré Souza Bissoli, Polyana Lima Meireles Dalpiaz, Izabela Facco Caliman |
| المصدر: | PLoS ONE, Vol 10, Iss 5, p e0127515 (2015) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Cardiac function curve, medicine.medical_specialty, Multidisciplinary, business.industry, Science, Phospholamban, Muscle hypertrophy, Contractility, medicine.anatomical_structure, Endocrinology, Ventricle, Internal medicine, medicine, Medicine, Orchiectomy, Systole, business, Testosterone, Research Article |
| الوصف: | BackgroundThere is growing interest in sex differences and RAS components. However, whether gender influences cardiac angiotensin I-converting enzyme (ACE) and angiotensin-converting enzyme 2 (ACE2) activity is still unknown. In the present work, we determined the relationship between ACE and ACE2 activity, left ventricular function and gender in spontaneously hypertensive rats (SHRs).Methodology/principal findingsTwelve-week-old female (F) and male (M) SHRs were divided into 2 experimental groups (n = 7 in each group): sham (S) and gonadectomized (G). Fifty days after gonadectomy, we measured positive and negative first derivatives (dP/dt maximum left ventricle (LV) and dP/dt minimum LV, respectively), hypertrophy (morphometric analysis) and ACE and ACE2 catalytic activity (fluorimetrically). Expression of calcium handling proteins was measured by western blot. Male rats exhibited higher cardiac ACE and ACE2 activity as well as hypertrophy compared to female rats. Orchiectomy decreased the activity of these enzymes and hypertrophy, while ovariectomy increased hypertrophy and ACE2, but did not change ACE activity. For cardiac function, the male sham group had a lower +dP/dt than the female sham group. After gonadectomy, the +dP/dt increased in males and reduced in females. The male sham group had a lower -dP/dt than the female group. After gonadectomy, the -dP/dt increased in the male and decreased in the female groups when compared to the sham group. No difference was observed among the groups in SERCA2a protein expression. Gonadectomy increased protein expression of PLB (phospholamban) and the PLB to SERCA2a ratio in female rats, but did not change in male rats.ConclusionOvariectomy leads to increased cardiac hypertrophy, ACE2 activity, PLB expression and PLB to SERCA2a ratio, and worsening of hemodynamic variables, whereas in males the removal of testosterone has the opposite effects on RAS components. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68f212f97a970521ffdcaaa8ccf8c955 https://doaj.org/article/fecdf46bfd4747f4ae6cbfd7be80ba6e |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....68f212f97a970521ffdcaaa8ccf8c955 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
|---|