Neuroprotective effect of bilberry extract in a murine model of photo-stressed retina
| العنوان: | Neuroprotective effect of bilberry extract in a murine model of photo-stressed retina |
|---|---|
| المؤلفون: | Norihiro Nagai, Hirohiko Kawashima, Hideto Osada, Kazuo Tsubota, Tomohiro Okamoto, Eriko Toda, Saori Kobayashi, Seiji Miyake, Yoko Ozawa |
| المصدر: | PLoS ONE, Vol 12, Iss 6, p e0178627 (2017) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Retinal degeneration, Male, Photoreceptors, Sensory Receptors, genetic structures, Vaccinium myrtillus, Social Sciences, lcsh:Medicine, Apoptosis, Retinal Pigment Epithelium, Endoplasmic Reticulum, Biochemistry, Photoreceptor cell, Antioxidants, chemistry.chemical_compound, Mice, Oxidative Damage, Animal Cells, Medicine and Health Sciences, Psychology, lcsh:Science, Neurons, Visual Impairments, Mice, Inbred BALB C, Multidisciplinary, Secretory Pathway, Cell Death, Retinal Degeneration, Animal Models, Endoplasmic Reticulum Stress, Cell biology, medicine.anatomical_structure, Experimental Organism Systems, Cell Processes, Models, Animal, Retinal Disorders, Sensory Perception, Cellular Types, Anatomy, Cellular Structures and Organelles, Research Article, Signal Transduction, Ocular Anatomy, Mouse Models, Research and Analysis Methods, Neuroprotection, Retina, 03 medical and health sciences, Model Organisms, Stress, Physiological, Ocular System, Retinitis pigmentosa, medicine, Electroretinography, Animals, Retinal pigment epithelium, Plant Extracts, lcsh:R, Biology and Life Sciences, Afferent Neurons, Retinal, Cell Biology, medicine.disease, Acetylcysteine, Ophthalmology, 030104 developmental biology, chemistry, Cellular Neuroscience, Unfolded protein response, lcsh:Q, sense organs, Reactive Oxygen Species, Neuroscience |
| الوصف: | Excessive exposure to light promotes degenerative and blinding retinal diseases such as age-related macular degeneration and retinitis pigmentosa. However, the underlying mechanisms of photo-induced retinal degeneration are not fully understood, and a generalizable preventive intervention has not been proposed. Bilberry extract is an antioxidant-rich supplement that ameliorates ocular symptoms. However, its effects on photo-stressed retinas have not been clarified. In this study, we examined the neuroprotective effects of bilberry extract against photo-stress in murine retinas. Light-induced visual function impairment recorded by scotopic and phototopic electroretinograms showing respective rod and cone photoreceptor function was attenuated by oral administration of bilberry extract through a stomach tube in Balb/c mice (750 mg/kg body weight). Bilberry extract also suppressed photo-induced apoptosis in the photoreceptor cell layer and shortening of the outer segments of rod and cone photoreceptors. Levels of photo-induced reactive oxygen species (ROS), oxidative and endoplasmic reticulum (ER) stress markers, as measured by real-time reverse transcriptase polymerase chain reaction, were reduced by bilberry extract treatment. Reduction of ROS by N-acetyl-L-cysteine, a well-known antioxidant also suppressed ER stress. Immunohistochemical analysis of activating transcription factor 4 expression showed the presence of ER stress in the retina, and at least in part, in Muller glial cells. The photo-induced disruption of tight junctions in the retinal pigment epithelium was also attenuated by bilberry extract, repressing an oxidative stress marker, although ER stress markers were not repressed. Our results suggest that bilberry extract attenuates photo-induced apoptosis and visual dysfunction most likely, and at least in part, through ROS reduction, and subsequent ER stress attenuation in the retina. This study can help understand the mechanisms of photo-stress and contribute to developing a new, potentially useful therapeutic approach using bilberry extract for preventing retinal photo-damage. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6875fbf0d838911792b6c92fa98e47ce http://europepmc.org/articles/PMC5453571?pdf=render |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6875fbf0d838911792b6c92fa98e47ce |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
|---|