Genotypic characterisation and antimicrobial resistance of Pseudomonas aeruginosa strains isolated from patients of different hospitals and medical centres in Poland
| العنوان: | Genotypic characterisation and antimicrobial resistance of Pseudomonas aeruginosa strains isolated from patients of different hospitals and medical centres in Poland |
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| المؤلفون: | Żaneta Krukowska, Joanna Jursa-Kulesza, Marcin Brzozowski, Katarzyna Galant, Danuta Kosik-Bogacka |
| المصدر: | BMC Infectious Diseases BMC Infectious Diseases, Vol 20, Iss 1, Pp 1-9 (2020) |
| بيانات النشر: | BioMed Central, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Genotyping, Genotyping Techniques, medicine.drug_class, 030106 microbiology, Antibiotics, Microbial Sensitivity Tests, Biology, medicine.disease_cause, Antimicrobial resistance, Population structure, lcsh:Infectious and parasitic diseases, Microbiology, 03 medical and health sciences, 0302 clinical medicine, Antibiotic resistance, Intensive care, Drug Resistance, Bacterial, Genotype, medicine, Pulsed-field gel electrophoresis, Humans, lcsh:RC109-216, Pseudomonas Infections, 030212 general & internal medicine, Cross Infection, Pseudomonas aeruginosa, PFGE, Antimicrobial, Hospitals, Anti-Bacterial Agents, Electrophoresis, Gel, Pulsed-Field, Intensive Care Units, Infectious Diseases, Poland, Research Article |
| الوصف: | Background Pseudomonas aeruginosa is a Gram-negative bacteria responsible for infections in immunocompromised patients and is one of the most common causes of nosocomial infections particularly in intensive care and burn units. We aimed to investigate the population structure of P. aeruginosa strains isolated from patients at different hospital wards. Methods: We analysed the possible presence of P. aeruginosa epidemic or endemic strains in hospitals of the selected region. A genotyping analysis was performed for P. aeruginosa isolates (n = 202) collected from patients of eleven hospitals in north-western Poland. Collections of P. aeruginosa were genotyped using pulsed-field gel electrophoresis (PFGE). Phenotypic screening for antibiotic susceptibility was performed for the common antimicrobial agents. Results Pseudomonas aeruginosa isolates were distributed among 116 different pulsotype groups. We identified 30 groups of clonally related strains, each containing from 2 to 17 isolates and typed the obtained 13 unique patterns, designated as A, D, E, J, K, M, N, Ó, P, T, X, AC, AD, and AH. The two largest clusters, D and E, contained 17 and 13 isolates, respectively. Strains of these groups were continuously isolated from patients at intensive care units and burn units, indicating transmission of these strains. Conclusions In this study, we demonstrate the clonal relatedness of P. aeruginosa strains and their constant exchange in hospitals over a period of 15 months. The obtained results indicate a predominantly non-clonal structure of P. aeruginosa. |
| اللغة: | English |
| تدمد: | 1471-2334 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67f5c07c1a28be6905d76c55050a7c84 http://europepmc.org/articles/PMC7507710 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....67f5c07c1a28be6905d76c55050a7c84 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712334 |
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