The CLEC-2–podoplanin axis controls the contractility of fibroblastic reticular cells and lymph node microarchitecture
| العنوان: | The CLEC-2–podoplanin axis controls the contractility of fibroblastic reticular cells and lymph node microarchitecture |
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| المؤلفون: | Max Darnell, Michael C. Carroll, Viviana Cremasco, Alvin Gogineni, Jillian L. Astarita, Jianxin Fu, Janice M Nieves-Bonilla, James R Peck, Matthew C. Woodruff, Burkhard Ludewig, Lijun Xia, David J. Mooney, Kai Song, Robby M. Weimer, Yuji Kondo, Lucas Onder, Shannon J. Turley |
| المصدر: | Nature immunology |
| بيانات النشر: | Springer Science and Business Media LLC, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Male, Pathology, medicine.medical_specialty, Mice, 129 Strain, Cell Survival, Pyridines, Immunology, Inflammation, Biology, Article, Contractility, Reticular cell, medicine, Animals, Immunology and Allergy, Lectins, C-Type, Enzyme Inhibitors, Phosphorylation, Lymph node, PDPN, Cytoskeleton, Mice, Knockout, Membrane Glycoproteins, Microscopy, Confocal, Fibroblasts, Amides, Specific Pathogen-Free Organisms, Cell biology, Mice, Inbred C57BL, medicine.anatomical_structure, Podoplanin, Reticular connective tissue, Female, Collagen, Lymph Nodes, Lymph, medicine.symptom |
| الوصف: | In lymph nodes, fibroblastic reticular cells (FRCs) form a collagen-based reticular network that supports migratory dendritic cells (DCs) and T cells and transports lymph. A hallmark of FRCs is their propensity to contract collagen, yet this function is poorly understood. Here we demonstrate that podoplanin (PDPN) regulates actomyosin contractility in FRCs. Under resting conditions, when FRCs are unlikely to encounter mature DCs expressing the PDPN receptor CLEC-2, PDPN endowed FRCs with contractile function and exerted tension within the reticulum. Upon inflammation, CLEC-2 on mature DCs potently attenuated PDPN-mediated contractility, which resulted in FRC relaxation and reduced tissue stiffness. Disrupting PDPN function altered the homeostasis and spacing of FRCs and T cells, which resulted in an expanded reticular network and enhanced immunity. |
| تدمد: | 1529-2916 1529-2908 |
| DOI: | 10.1038/ni.3035 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67299cb13a0f0dfce27060a6f6ffc680 https://doi.org/10.1038/ni.3035 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....67299cb13a0f0dfce27060a6f6ffc680 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15292916 15292908 |
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| DOI: | 10.1038/ni.3035 |