Consequences of producing DNA gyrase from a synthetic gyrBA operon in Salmonella enterica serovar Typhimurium
| العنوان: | Consequences of producing DNA gyrase from a synthetic gyrBA operon in Salmonella enterica serovar Typhimurium |
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| المؤلفون: | German Pozdeev, Charles J. Dorman, Aalap Mogre |
| المصدر: | Molecular Microbiology |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | DNA, Bacterial, Salmonella typhimurium, Transcription, Genetic, Operon, Virulence, gyrA, gyrB, Salmonella enterica serovar Typhimurium, Biology, Cell morphology, Microbiology, Genome, DNA gyrase, 03 medical and health sciences, chemistry.chemical_compound, Bacterial Proteins, heterocyclic compounds, Molecular Biology, Gene, Research Articles, 030304 developmental biology, Genetics, 0303 health sciences, Base Sequence, 030306 microbiology, DNA, Superhelical, Macrophages, Membrane Proteins, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, bacterial infections and mycoses, SPI‐2, DNA supercoiling, SPI‐1, chemistry, Salmonella enterica, DNA supercoil, bacteria, Type II topoisomerase, DNA, Genome, Bacterial, Research Article |
| الوصف: | DNA gyrase is an essential type II topoisomerase that is composed of two subunits, GyrA and GyrB, and has an A2B2 structure. Although the A and B subunits are required in equal proportions to form DNA gyrase, the gyrA and gyrB genes that encode them in Salmonella (and in many other bacteria) are at separate locations on the chromosome, are under separate transcriptional control, and are present in different copy numbers in rapidly growing bacteria. In wild‐type Salmonella, gyrA is near the chromosome's replication terminus, while gyrB is near the origin. We generated a synthetic gyrBA operon at the oriC‐proximal location of gyrB to test the significance of the gyrase gene position for Salmonella physiology. Although the strain producing gyrase from an operon had a modest alteration to its DNA supercoiling set points, most housekeeping functions were unaffected. However, its SPI‐2 virulence genes were expressed at a reduced level and its survival was reduced in macrophage. Our data reveal that the horizontally acquired SPI‐2 genes have a greater sensitivity to disturbance of DNA topology than the core genome and we discuss its significance in the context of Salmonella genome evolution and the gyrA and gyrB gene arrangements found in other bacteria. DNA gyrase is an essential topoisomerase in bacteria that plays critical roles in the management of nucleoid structure, DNA replication, transcription and recombination. It is also a major drug target. The gyrA and gyrB genes that encode the two subunits of DNA gyrase are located at widely separated sites on the chromosome of Salmonella and other bacteria. In contrast, many bacteria, such as Mycobacterium tuberculosis, Bacillus subtilis and Listeria monocytogenes, maintain the gyrA and gyrB genes in an operon. We report an investigation of the effects on Salmonella physiology and virulence of producing DNA gyrase from an operon, and the patterns of gyrase gene arrangements found in a wide range of bacteria. We believe that our findings will be of interest to those studying DNA gyrase, bacterial nucleoid architecture, gene regulation and Salmonella virulence. Our work can also assist those interested in the application of synthetic biology to bacterial chromosome design. |
| تدمد: | 1365-2958 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6664e90fd326e93f85bc8cf1eeac8e8b https://pubmed.ncbi.nlm.nih.gov/33539568 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6664e90fd326e93f85bc8cf1eeac8e8b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652958 |
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