Keratinocyte Cytotoxicity of Peracetic Acid Used as Sterilizing Agent for Implant Scaffolds
| العنوان: | Keratinocyte Cytotoxicity of Peracetic Acid Used as Sterilizing Agent for Implant Scaffolds |
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| المؤلفون: | Marcelo Lazzaron Lamers, Rodrigo Alex Arthur, Artur Ferronato Soto, Cristiane Machado Mengatto, Myriam Pereira Kapczinski |
| المصدر: | Annals of Plastic Surgery. 83:99-103 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Keratinocytes, Prosthesis-Related Infections, Cell, 030230 surgery, Risk Assessment, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Anti-Infective Agents, Reference Values, Peracetic acid, medicine, Humans, Cytotoxic T cell, Peracetic Acid, Viability assay, Cytotoxicity, Cells, Cultured, Analysis of Variance, Cell Death, Dose-Response Relationship, Drug, business.industry, Sterilization, Biomaterial, Prostheses and Implants, Molecular biology, HaCaT, medicine.anatomical_structure, chemistry, Case-Control Studies, 030220 oncology & carcinogenesis, Surgery, Keratinocyte, business |
| الوصف: | Peracetic acid (PAA) has been used to sterilize biomaterial scaffolds and allografts before their implantation. Although the antimicrobial effectiveness of PAA is widely known, there are no studies investigating its cytotoxicity on keratinocytes. This study aimed to investigate the cytotoxicity of PAA concentrations on keratinocytes by growing HaCaT cells in culture medium. Different concentrations of PAA (control-untreated, 0.01, 0.1, 1, 10, 100, 200, 400, 800, 1200, 1600, and 2000 ppm) were added to the culture wells and allowed to be in direct contact with cells for up to 24 hours. Cytotoxicity was quantitatively and qualitatively determined by cell viability assay and analysis of morphological changes. Statistical analysis was performed with 1-way analysis of variance and Tukey test at 5% significance. Cells treated with 0.01 and 0.1 ppm followed the same morphological pattern of untreated cells, whereas cells treated with 1.0 ppm presented about 20% of floating cells and dark cytoplasmic granules. More than 50% of the cells treated with 10 and 100 ppm were destroyed, whereas the attached ones showed unclear and interrupted cell membranes. Concentrations of 1 ppm or greater had less than 64.4% of viable cells compared with the control group. This study concluded that exposure of keratinocytes to concentrations of 1 ppm or greater of PAA resulted in strong cytotoxic effects. |
| تدمد: | 1536-3708 0148-7043 |
| DOI: | 10.1097/sap.0000000000001967 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66463e5cc46ecd3a8e2a4670a91f1e5d https://doi.org/10.1097/sap.0000000000001967 |
| رقم الانضمام: | edsair.doi.dedup.....66463e5cc46ecd3a8e2a4670a91f1e5d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15363708 01487043 |
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| DOI: | 10.1097/sap.0000000000001967 |