Cellular and karyotypic characterization of two doxorubicin resistant cell lines isolated from the same parental human leukemia cell line
| العنوان: | Cellular and karyotypic characterization of two doxorubicin resistant cell lines isolated from the same parental human leukemia cell line |
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| المؤلفون: | Mario de Luise, John D. Parkin, Shelagh E. L. Mirski, Phillip Kantharidis, Vickie Vrazas, Gabrielle Nadalin, Susan P.C. Cole, Xiu F. Hu, Lynda J. Campbell, John Zalcberg, Dominic M. Wall |
| المصدر: | International Journal of Cancer. 57:522-528 |
| بيانات النشر: | Wiley, 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | Cancer Research, Microgram, Immunoblotting, T-cell leukemia, Drug Resistance, Antineoplastic Agents, Biology, Models, Biological, Antibodies, Tumor Cells, Cultured, medicine, Humans, Cytotoxic T cell, ATP Binding Cassette Transporter, Subfamily B, Member 1, Etoposide, P-glycoprotein, Chromosome 7 (human), Genetics, Leukemia, Membrane Glycoproteins, Gene Amplification, Flow Cytometry, Molecular biology, Multiple drug resistance, DNA Topoisomerases, Type II, Phenotype, Oncology, Doxorubicin, Cell culture, Karyotyping, biology.protein, RNA, Carrier Proteins, medicine.drug |
| الوصف: | Separate mechanisms underlying the multidrug resistant (MDR) phenotype were identified in 2 independent approaches to select tumour cells resistant to low concentrations of doxorubicin (Dox) from the sensitive T cell leukemia cell line CCRF-CEM. The CEM/A7 cell line was selected at an initial concentration of 0.005 microgram/ml of Dox and maintained at 0.07 microgram/ml. In contrast, the CEM/A5 line was selected using an initial concentration of 0.01 microgram/ml and maintained in Dox at a concentration of 0.05 microgram/ml. P-glycoprotein expression was demonstrated in the CEM/A7 line but not the CEM/A5 line. Amplification of the mdrI gene was not observed in the CEM/A7 cell line. Both cell lines showed cross-resistance to a number of structurally unrelated cytotoxic drugs including anthracyclines and etoposide (VP-16), although only the CEM/A7 line was cross resistant to Vinca alkaloids. Immunoblots of total cell lysates of the CEM/A5 line have revealed almost undetectable levels of topoisomerase II alpha and beta in this line. Cytogenetic analyses of both lines revealed numerous karyotypic abnormalities which were present in the parental cell line as well as both resistant cell lines. The CEM/A7 line also demonstrated a duplication of part of the long arm of chromosome 7 which included the region containing the mdrI gene, a finding not seen in the parental or CEM/A5 line. CEM/A5, however, demonstrated an abnormality of chromosome 7, outside the region of the mdrI gene, and it also contained a deletion of the short arm of chromosome 2. Abnormalities in this latter region of genome have been associated with non-P-glycoprotein-mediated MDR. |
| تدمد: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.2910570414 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65f721e8b352f735bb93472083fb497f https://doi.org/10.1002/ijc.2910570414 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....65f721e8b352f735bb93472083fb497f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970215 00207136 |
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| DOI: | 10.1002/ijc.2910570414 |