Inactivation of phoPQ genes attenuates Salmonella Gallinarum biovar Gallinarum to susceptible chickens
| العنوان: | Inactivation of phoPQ genes attenuates Salmonella Gallinarum biovar Gallinarum to susceptible chickens |
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| المؤلفون: | Lucas Bocchini Rodrigues Alves, Oliveiro Caetano de Freitas Neto, Diego Felipe Alves Batista, Adriana Maria de Almeida, Andrei Itajahy Secundo de Souza, Fernanda de Oliveira Barbosa, Marcela da Silva Rubio, Angelo Berchieri Junior, Paul A. Barrow |
| المساهمون: | Universidade Estadual Paulista (Unesp), Federal University of Paraiba, School of Veterinary Medicine and Science |
| المصدر: | Brazilian Journal of Microbiology, Volume: 49, Issue: 3, Pages: 601-606, Published: SEP 2018 Brazilian Journal of Microbiology, Vol 49, Iss 3, Pp 601-606 Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Brazilian Journal of Microbiology v.49 n.3 2018 Brazilian Journal of Microbiology Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
| بيانات النشر: | Sociedade Brasileira de Microbiologia, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Salmonella, Biovar, Veterinary Microbiology, 030106 microbiology, Antimicrobial peptides, lcsh:QR1-502, Virulence, Spleen, Fowl typhoid, medicine.disease_cause, Microbiology, lcsh:Microbiology, 03 medical and health sciences, Bacterial Proteins, medicine, Animals, Gene Silencing, Adaptation, Gene, Pathogen, Poultry Diseases, Salmonella Infections, Animal, biology, Salmonella enterica, biology.organism_classification, Antimicrobial, 030104 developmental biology, medicine.anatomical_structure, Mutation, Female, Chickens |
| الوصف: | Made available in DSpace on 2018-12-11T16:52:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-07-01. Added 1 bitstream(s) on 2021-07-15T15:21:28Z : No. of bitstreams: 1 S1517-83822018000300601.pdf: 692251 bytes, checksum: b1b3eaf5bece488e7ed9ca1ca902e1c6 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Salmonella Gallinarum is a host-restrict pathogen that causes fowl typhoid, a severe systemic disease that is one of the major concerns to the poultry industry worldwide. When infecting the bird, SG makes use of evasion mechanisms to survive and to replicate within macrophages. In this context, phoPQ genes encode a two-component regulatory system (PhoPQ) that regulates virulence genes responsible for adaptation of Salmonella spp. to antimicrobial factors such as low pH, antimicrobial peptides and deprivation of bivalent cations. The role of the mentioned genes to SG remains to be investigated. In the present study a phoPQ-depleted SG strain (SG ΔphoPQ) was constructed and its virulence assessed in twenty-day-old laying hens susceptible to fowl typhoid. SG ΔphoPQ did cause neither clinical signs nor mortality in birds orally challenged, being non-pathogenic. Furthermore, this strain was not recovered from livers or spleens. On the other hand, chickens challenged subcutaneously with the mutant strain had discreet to moderate pathological changes and also low bacterial counts in liver and spleen tissues. These findings show that SG ΔphoPQ is attenuated to susceptible chickens and suggest that these genes are important during chicken infection by SG. São Paulo State University (Unesp) School of Agricultural and Veterinary Sciences Post Graduate Program in Agricultural and Livestock Microbiology Federal University of Paraiba Department of Veterinary Sciences University of Nottingham School of Veterinary Medicine and Science, Sutton Bonington São Paulo State University (Unesp) School of Agricultural and Veterinary Sciences Post Graduate Program in Agricultural and Livestock Microbiology FAPESP: 2016/10369-0 |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 1517-8382 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64b2a37093712ac3c80bec456bb1b618 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300601&lng=en&tlng=en |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....64b2a37093712ac3c80bec456bb1b618 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15178382 |
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