Transcriptomic Analysis in Liquid Biopsy Identifies Circulating PCTAIRE-1 mRNA as a Biomarker in NSCLC
| العنوان: | Transcriptomic Analysis in Liquid Biopsy Identifies Circulating PCTAIRE-1 mRNA as a Biomarker in NSCLC |
|---|---|
| المؤلفون: | Chun-Liang Shih, Chih-Wei Wang, John Wen-Cheng Chang, Chih-Liang Wang, Ji-Dung Luo, Chiuan-Chian Chiou, Jia-Juan Hsieh, Chia-Jung Yu |
| المصدر: | Cancer Genomics - Proteomics. 17:91-100 |
| بيانات النشر: | Anticancer Research USA Inc., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, Male, Cancer Research, Lung Neoplasms, Microarray, Biochemistry, Transcriptome, 03 medical and health sciences, 0302 clinical medicine, Carcinoma, Non-Small-Cell Lung, Antineoplastic Combined Chemotherapy Protocols, Biomarkers, Tumor, Genetics, Humans, Medicine, RNA, Messenger, Liquid biopsy, Molecular Biology, Gene, Aged, Aged, 80 and over, Messenger RNA, business.industry, Liquid Biopsy, RNA, Middle Aged, Prognosis, Cyclin-Dependent Kinases, Gene Expression Regulation, Neoplastic, Survival Rate, ROC Curve, Case-Control Studies, 030220 oncology & carcinogenesis, Cancer research, Female, Cancer biomarkers, DNA microarray, business, Follow-Up Studies, Research Article |
| الوصف: | Background/aim Circulating mRNA can be a useful source of cancer biomarkers. We took advantage of direct transcriptomic analysis in plasma RNA to identify novel mRNA markers for non-small cell lung cancer (NSCLC). Patients and methods Plasma RNA from NSCLC patients and healthy individuals was profiled with cDNA-mediated annealing, selection, extension and ligation (DASL) microarrays. The microarray results were further validated in plasma RNA. Results Through RNA profiling and online database mining, four gene transcripts were filtered as candidate markers of NSCLC. After validation, the PCTAIRE-1 transcript was identified as a circulating mRNA marker. The diagnostic potential of PCTAIRE-1 was evaluated by receiver operating characteristic curve analysis, which gave a sensitivity and specificity of 60% and 85%, respectively. In addition, high plasma PCTK1 levels were also correlated with poor progression-free survival (p=0.008). Conclusion Circulating mRNA can be profiled with the DASL assay. From the profile, PCTAIRE-1 RNA in the plasma we discovered as a novel diagnostic/prognostic biomarker and an indicator of poor survival in NSCLC patients. |
| تدمد: | 1790-6245 1109-6535 |
| DOI: | 10.21873/cgp.20170 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6320a388e22aabe45d6b63ca313d7452 https://doi.org/10.21873/cgp.20170 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6320a388e22aabe45d6b63ca313d7452 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17906245 11096535 |
|---|---|
| DOI: | 10.21873/cgp.20170 |