A method to identify trace sulfated IgG N-glycans as biomarkers for rheumatoid arthritis
| العنوان: | A method to identify trace sulfated IgG N-glycans as biomarkers for rheumatoid arthritis |
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| المؤلفون: | Liang Liu, Tian-Tian Tong, Hai-Hui Huang, Qiong Meng, Xing Zeng, Wei-Na Gao, Rudolf Grimm, Hua Ye, Min Jiang, Ju Liu, Hao Huang, Shibo Jiang, Zhi-Hong Jiang, Zhan-Guo Li, Yong Liang, Jing-Rong Wang, Stephanie Lee, Lee-Fong Yau |
| المصدر: | Nature Communications, Vol 8, Iss 1, Pp 1-14 (2017) Nature Communications |
| بيانات النشر: | Nature Portfolio, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Glycan, Glycosylation, Science, General Physics and Astronomy, Arthritis, Peptides, Cyclic, 01 natural sciences, Article, General Biochemistry, Genetics and Molecular Biology, Immunoglobulin G, Arthritis, Rheumatoid, Glycomics, 03 medical and health sciences, chemistry.chemical_compound, Polysaccharides, Rheumatoid Factor, medicine, Humans, Rheumatoid factor, lcsh:Science, Aged, Multidisciplinary, biology, Sulfates, 010401 analytical chemistry, fungi, food and beverages, Acetylation, General Chemistry, Middle Aged, medicine.disease, 0104 chemical sciences, 030104 developmental biology, chemistry, Case-Control Studies, Rheumatoid arthritis, Immunology, biology.protein, Female, lcsh:Q, Antibody, Protein Processing, Post-Translational, Biomarkers |
| الوصف: | N-linked glycans on immunoglobulin G (IgG) have been associated with pathogenesis of diseases and the therapeutic functions of antibody-based drugs; however, low-abundance species are difficult to detect. Here we show a glycomic approach to detect these species on human IgGs using a specialized microfluidic chip. We discover 20 sulfated and 4 acetylated N-glycans on IgGs. Using multiple reaction monitoring method, we precisely quantify these previously undetected low-abundance, trace and even ultra-trace N-glycans. From 277 patients with rheumatoid arthritis (RA) and 141 healthy individuals, we also identify N-glycan biomarkers for the classification of both rheumatoid factor (RF)-positive and negative RA patients, as well as anti-citrullinated protein antibodies (ACPA)-positive and negative RA patients. This approach may identify N-glycosylation-associated biomarkers for other autoimmune and infectious diseases and lead to the exploration of promising glycoforms for antibody therapeutics. Post-translational modifications can affect antibody function in health and disease, but identification of all variants is difficult using existing technologies. Here the authors develop a microfluidic method to identify and quantify low-abundance IgG N-glycans and show some of these IgGs can be used as biomarkers for rheumatoid arthritis. |
| اللغة: | English |
| تدمد: | 2041-1723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62d21d93bb47ae692c63601b16cc3ffa https://doaj.org/article/ca2ca638cde14bb8b892f0ac694cda79 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....62d21d93bb47ae692c63601b16cc3ffa |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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