التفاصيل البيبلوغرافية
| العنوان: |
Transcriptomic response of maize primary roots to low temperatures at seedling emergence |
| المؤلفون: |
Jim Grant, Susanne Barth, Mauro Di Fenza, Bridget V. Hogg |
| المساهمون: |
Department of Agriculture, Food and the Marine, RSF 07 501 |
| المصدر: |
PeerJ
PeerJ, Vol 5, p e2839 (2017) |
| بيانات النشر: |
PeerJ, 2016. |
| سنة النشر: |
2016 |
| مصطلحات موضوعية: |
0106 biological sciences, 0301 basic medicine, Germplasm, lcsh:Medicine, Plant Science, Biology, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, Transcriptome, 03 medical and health sciences, Temperate climate, Low temperature, Agricultural Science, Gene, General Neuroscience, lcsh:R, fungi, food and beverages, General Medicine, biology.organism_classification, Chilling tolerance, Roots, Zea mays, Maize, 030104 developmental biology, Agronomy, Seedling, Gene pool, Adaptation, General Agricultural and Biological Sciences, Cold stress, 010606 plant biology & botany |
| الوصف: |
BackgroundMaize (Zea mays) is a C4tropical cereal and its adaptation to temperate climates can be problematic due to low soil temperatures at early stages of establishment.MethodsIn the current study we have firstly investigated the physiological response of twelve maize varieties, from a chilling condition adapted gene pool, to sub-optimal growth temperature during seedling emergence. To identify transcriptomic markers of cold tolerance in already adapted maize genotypes, temperature conditions were set below the optimal growth range in both control and low temperature groups. The conditions were as follows; control (18 °C for 16 h and 12 °C for 8 h) and low temperature (12 °C for 16 h and 6 °C for 8 h). Four genotypes were identified from the condition adapted gene pool with significant contrasting chilling tolerance.ResultsPicker and PR39B29 were the more cold-tolerant lines and Fergus and Codisco were the less cold-tolerant lines. These four varieties were subjected to microarray analysis to identify differentially expressed genes under chilling conditions. Exposure to low temperature during establishment in the maize varieties Picker, PR39B29, Fergus and Codisco, was reflected at the transcriptomic level in the varieties Picker and PR39B29. No significant changes in expression were observed in Fergus and Codisco following chilling stress. A total number of 64 genes were differentially expressed in the two chilling tolerant varieties. These two varieties exhibited contrasting transcriptomic profiles, in which only four genes overlapped.DiscussionWe observed that maize varieties possessing an enhanced root growth ratio under low temperature were more tolerant, which could be an early and inexpensive measure for germplasm screening under controlled conditions. We have identified novel cold inducible genes in an already adapted maize breeding gene pool. This illustrates that further varietal selection for enhanced chilling tolerance is possible in an already preselected gene pool. |
| اللغة: |
English |
| DOI: |
10.7287/peerj.preprints.2372v1 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61e5642ebf2f8147754aeababde7dc7f |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....61e5642ebf2f8147754aeababde7dc7f |
| قاعدة البيانات: |
OpenAIRE |