Applicability of an innovative steroid-profiling method to determine synthetic growth promoter abuse in cattle
| العنوان: | Applicability of an innovative steroid-profiling method to determine synthetic growth promoter abuse in cattle |
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| المؤلفون: | Marco Blokland, Saskia S. Sterk, E.F. van Tricht, L.A. van Ginkel |
| المصدر: | Journal of Steroid Biochemistry and Molecular Biology, 174, 265-275 Journal of Steroid Biochemistry and Molecular Biology 174 (2017) |
| بيانات النشر: | Elsevier BV, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Monsteradministratie & Coördinatie, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Clinical Biochemistry, Diethylstilbestrol, UHPLC–MS/MS, Steroid profiling, Urine, 01 natural sciences, Biochemistry, BU Dierbehandelingsmiddelen, BU Veterinary Drugs, Steroid, 03 medical and health sciences, Anabolic Agents, Endocrinology, Growth promoters, Tandem Mass Spectrometry, medicine, Animals, Clenbuterol, Estrogens, Non-Steroidal, Synthetic Steroids, Molecular Biology, Chromatography, High Pressure Liquid, Stanozolol, Synthetic steroids, Chromatography, Chemistry, 010401 analytical chemistry, Normal population, Cell Biology, 0104 chemical sciences, 030104 developmental biology, Steroidogenesis, Molecular Medicine, Cattle, Steroids, After treatment, medicine.drug |
| الوصف: | A robust LC–MS/MS method was developed to quantify a large number of phase I and phase II steroids in urine. The decision limit is for most compounds lower than 1 ng ml−1 with a measurement uncertainty smaller than 30%. The method is fully validated and was applied to assess the influence of administered synthetic steroids and beta-agonists on the steroidogenesis. From three animal experiments, clenbuterol, diethylstilbestrol and stanozolol, the steroid profiles in urine of bovine animals were compared before and after treatment. It was demonstrated that the steroid profiles were altered due to these treatments. A predictive multivariate model was built to identify deviations from normal population steroid profiles. The abuse of synthetic steroids can be detected in urine samples from bovine animals using this model. The samples from the animal experiments were randomly analysed using this method and predictive model. It was shown that these samples were predicted correctly in the exogenous steroids group. |
| وصف الملف: | application/pdf |
| تدمد: | 0960-0760 |
| DOI: | 10.1016/j.jsbmb.2017.10.007 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60a8278a71ebdb7f047a0158655cd6b4 https://doi.org/10.1016/j.jsbmb.2017.10.007 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....60a8278a71ebdb7f047a0158655cd6b4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09600760 |
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| DOI: | 10.1016/j.jsbmb.2017.10.007 |