Fluorescent nanoparticle‐mediated semiquantitative MYC protein expression analysis in morphologically diffuse large B‐cell lymphoma
العنوان: | Fluorescent nanoparticle‐mediated semiquantitative MYC protein expression analysis in morphologically diffuse large B‐cell lymphoma |
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المؤلفون: | Morihiro Higashi, Natsuko Takayanagi, Michihide Tokuhira, Yuka Tanaka, Jun Kikuchi, Takahisa Yamashita, Shuji Momose, Tomoe Anan, Masahiro Kizaki, Jun-ichi Tamaru |
المصدر: | Pathology International. 71:594-603 |
بيانات النشر: | Wiley, 2021. |
سنة النشر: | 2021 |
مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Pathology, medicine.medical_specialty, 3,3'-Diaminobenzidine, Biology, Pathology and Forensic Medicine, Proto-Oncogene Proteins c-myc, Young Adult, 03 medical and health sciences, 0302 clinical medicine, immune system diseases, hemic and lymphatic diseases, medicine, Humans, Gene, In Situ Hybridization, Fluorescence, Aged, Gene Rearrangement, Luminescent Agents, medicine.diagnostic_test, General Medicine, Middle Aged, Prognosis, medicine.disease, BCL6, Immunohistochemistry, Fluorescence, Lymphoma, 030104 developmental biology, 030220 oncology & carcinogenesis, Protein Expression Analysis, Cancer research, Nanoparticles, Female, Lymphoma, Large B-Cell, Diffuse, Diffuse large B-cell lymphoma, Fluorescence in situ hybridization |
الوصف: | The current World Health Organization (WHO) classification defines a new disease entity of high-grade B-cell lymphoma with MYC and BCL2 and/or BCL6 rearrangements, making fluorescence in situ hybridization (FISH) screening for these genes mandatory. In addition, the prognostic significance of MYC expression was reported, with a cut-off value of 40%. However, interobserver discrepancies arise due to the heterogeneous intensity of MYC expression by immunohistochemistry. Moreover, a cut-off value of positivity for MYC protein in diffuse large B-cell lymphoma (DLBCL) varies among studies at present. Here, we applied a high-sensitivity semiquantitative immunohistochemical technique using fluorescent nanoparticles called phosphor-integrated dots (PID) to evaluate the MYC expression in 50 de novo DLBCL cases, and compared it with the conventional diaminobenzidine (DAB)-developing system. The high MYC expression detected by the PID-mediated system predicted poor overall survival in DLBCL patients. However, we found no prognostic value of MYC protein expression for any cut-off value by the DAB-developing system, even if the intensity was considered. These results indicate that the precise evaluation of MYC protein expression can clarify the prognostic values in DLBCL, irrespective of MYC rearrangement. |
تدمد: | 1440-1827 1320-5463 |
DOI: | 10.1111/pin.13131 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6039e4246d6d9df0865aee9c0bdba183 https://doi.org/10.1111/pin.13131 |
Rights: | CLOSED |
رقم الانضمام: | edsair.doi.dedup.....6039e4246d6d9df0865aee9c0bdba183 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 14401827 13205463 |
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DOI: | 10.1111/pin.13131 |