Fluorescent nanoparticle‐mediated semiquantitative MYC protein expression analysis in morphologically diffuse large B‐cell lymphoma

التفاصيل البيبلوغرافية
العنوان: Fluorescent nanoparticle‐mediated semiquantitative MYC protein expression analysis in morphologically diffuse large B‐cell lymphoma
المؤلفون: Morihiro Higashi, Natsuko Takayanagi, Michihide Tokuhira, Yuka Tanaka, Jun Kikuchi, Takahisa Yamashita, Shuji Momose, Tomoe Anan, Masahiro Kizaki, Jun-ichi Tamaru
المصدر: Pathology International. 71:594-603
بيانات النشر: Wiley, 2021.
سنة النشر: 2021
مصطلحات موضوعية: Adult, Male, 0301 basic medicine, Pathology, medicine.medical_specialty, 3,3'-Diaminobenzidine, Biology, Pathology and Forensic Medicine, Proto-Oncogene Proteins c-myc, Young Adult, 03 medical and health sciences, 0302 clinical medicine, immune system diseases, hemic and lymphatic diseases, medicine, Humans, Gene, In Situ Hybridization, Fluorescence, Aged, Gene Rearrangement, Luminescent Agents, medicine.diagnostic_test, General Medicine, Middle Aged, Prognosis, medicine.disease, BCL6, Immunohistochemistry, Fluorescence, Lymphoma, 030104 developmental biology, 030220 oncology & carcinogenesis, Protein Expression Analysis, Cancer research, Nanoparticles, Female, Lymphoma, Large B-Cell, Diffuse, Diffuse large B-cell lymphoma, Fluorescence in situ hybridization
الوصف: The current World Health Organization (WHO) classification defines a new disease entity of high-grade B-cell lymphoma with MYC and BCL2 and/or BCL6 rearrangements, making fluorescence in situ hybridization (FISH) screening for these genes mandatory. In addition, the prognostic significance of MYC expression was reported, with a cut-off value of 40%. However, interobserver discrepancies arise due to the heterogeneous intensity of MYC expression by immunohistochemistry. Moreover, a cut-off value of positivity for MYC protein in diffuse large B-cell lymphoma (DLBCL) varies among studies at present. Here, we applied a high-sensitivity semiquantitative immunohistochemical technique using fluorescent nanoparticles called phosphor-integrated dots (PID) to evaluate the MYC expression in 50 de novo DLBCL cases, and compared it with the conventional diaminobenzidine (DAB)-developing system. The high MYC expression detected by the PID-mediated system predicted poor overall survival in DLBCL patients. However, we found no prognostic value of MYC protein expression for any cut-off value by the DAB-developing system, even if the intensity was considered. These results indicate that the precise evaluation of MYC protein expression can clarify the prognostic values in DLBCL, irrespective of MYC rearrangement.
تدمد: 1440-1827
1320-5463
DOI: 10.1111/pin.13131
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6039e4246d6d9df0865aee9c0bdba183
https://doi.org/10.1111/pin.13131
Rights: CLOSED
رقم الانضمام: edsair.doi.dedup.....6039e4246d6d9df0865aee9c0bdba183
قاعدة البيانات: OpenAIRE
الوصف
تدمد:14401827
13205463
DOI:10.1111/pin.13131