Chronic ethanol consumption induces erectile dysfunction: Role of oxidative stress
| العنوان: | Chronic ethanol consumption induces erectile dysfunction: Role of oxidative stress |
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| المؤلفون: | Bruno Spinosa De Martinis, Jaqueline J. Muniz, Carlos R. Tirapelli, Fernando S. Carneiro, Letícia N. Leite |
| المصدر: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Contraction (grammar), ENDOTELINAS (FARMACOLOGIA), Vasodilation, medicine.disease_cause, Thiobarbituric Acid Reactive Substances, General Biochemistry, Genetics and Molecular Biology, Superoxide dismutase, chemistry.chemical_compound, Erectile Dysfunction, Superoxides, Internal medicine, medicine, TBARS, Animals, Arterial Pressure, General Pharmacology, Toxicology and Pharmaceutics, Rats, Wistar, chemistry.chemical_classification, Reactive oxygen species, biology, Endothelin-1, Ethanol, Superoxide, Central Nervous System Depressants, General Medicine, Hydrogen Peroxide, Catalase, Rats, Oxidative Stress, Endocrinology, chemistry, Cyclooxygenase 2, biology.protein, 1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt, Cyclooxygenase 1, Reactive Oxygen Species, Oxidative stress, Muscle Contraction, Penis |
| الوصف: | Aims Investigate the effects of chronic ethanol consumption on erectile function and on the corpus cavernosum (CC) reactivity to endothelin-1 (ET-1). Main methods Male Wistar rats were treated with ethanol (20% v / v ) for six weeks. Key findings Ethanol-treated rats showed impaired erectile function represented by decreased intracavernosal pressure/mean arterial pressure (ICP/MAP) responses. Ethanol consumption increased the contractile response induced by ET-1 in the isolated CC. Tiron increased ET-1-induced contraction in CC from control and ethanol-treated rats. No differences in the maximal contraction to ET-1 were observed after incubation of CC with PEG-catalase. SC560 and SC236 increased ET-1-induced contraction in CC from ethanol-treated rats. Y27632 reduced the contraction induced by ET-1 in CC from control and ethanol-treated rats. Ethanol increased plasma TBARS, superoxide anion (O 2 − ) levels and intracellular reactive oxygen species (ROS) generation in the rat CC. Reduced hydrogen peroxide (H 2 O 2 ) levels in CC and increased catalase (CAT) activity in plasma and CC were detected after treatment with ethanol. Ethanol decreased superoxide dismutase (SOD) activity in the rat CC. Increased expression of COX-1 was observed in CC from ethanol-treated rats. Treatment with ethanol decreased COX-2 expression but did not alter the expression of Nox1, RhoA and p-RhoA (ser 188 ) in the rat CC. Significance The major new findings of our study are that ethanol consumption induces erectile dysfunction (ED) and increases the contraction induced by ET-1 in the rat CC by a mechanism that involves decreased generation of H 2 O 2 and vasodilator prostanoids as well as increased activation of the RhoA/Rho-kinase pathway. |
| تدمد: | 1879-0631 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ff4f86d1c87e7de94d60cdf54089020 https://pubmed.ncbi.nlm.nih.gov/26407475 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5ff4f86d1c87e7de94d60cdf54089020 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18790631 |
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