Drug resistance to sulphadoxine-pyrimethamine in Plasmodium falciparum malaria in Mlimba, Tanzania
| العنوان: | Drug resistance to sulphadoxine-pyrimethamine in Plasmodium falciparum malaria in Mlimba, Tanzania |
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| المؤلفون: | Allen L Malisa, B. M. Mutayoba, Erasto V. Mbugi, Hassan Mshinda, Thomas B. Nyambo, ST Balthazary |
| المصدر: | Malaria Journal Malaria Journal, 5 Malaria Journal, Vol 5, Iss 1, p 94 (2006) Malaria Journal 5 (2006) |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Male, dihydropteroate-synthetase genes, medicine.medical_treatment, Drug Resistance, DHPS, Drug resistance, in-vivo, Polymerase Chain Reaction, Tanzania, Parasitic Sensitivity Tests, Malaria, Falciparum, education.field_of_study, biology, antifolate resistance, Drug Combinations, synthase, Infectious Diseases, Pyrimethamine, dihydrofolate-reductase, Child, Preschool, Female, Drugs, Medical Supplies & Logistics, Polymorphism, Restriction Fragment Length, medicine.drug, lcsh:Arctic medicine. Tropical medicine, molecular markers, lcsh:RC955-962, Sulfadoxine, Population, Plasmodium falciparum, Celbiologie en Immunologie, proguanil resistance, lcsh:Infectious and parasitic diseases, chlorproguanil-dapsone, Antimalarials, parasitic diseases, medicine, Animals, Humans, Point Mutation, lcsh:RC109-216, education, treatment failure, Dihydropteroate Synthase, Research, Infant, mutations, biology.organism_classification, medicine.disease, Virology, Tetrahydrofolate Dehydrogenase, Cell Biology and Immunology, WIAS, Parasitology, Dihydropteroate synthase, Malaria |
| الوصف: | Background Sulphadoxine-pyrimethamine (SP) has been and is currently used for treatment of uncomplicated Plasmodium falciparum malaria in many African countries. Nevertheless, the response of parasites to SP treatment has shown significant variation between individuals. Methods The genes for dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) were used as markers, to investigate parasite resistance to SP in 141 children aged less than 5 years. Parasite DNA was extracted by Chelex method from blood samples collected and preserved on filter papers. Subsequently, polymerase chain reaction (PCR) and restriction fragment length polymorphism (PCR-RFLP) were applied to detect the SP resistance-associated point mutations on dhfr and dhps. Commonly reported point mutations at codons 51, 59, 108 and 164 in the dhfr and codons 437, 540 and 581 in the dhps domains were examined. Results Children infected with parasites harbouring a range of single to quintuple dhfr/dhps mutations were erratically cured with SP. However, the quintuple dhfr/dhps mutant genotypes were mostly associated with treatment failures. High proportion of SP resistance-associated point mutations was detected in this study but the adequate clinical response (89.4%) observed clinically at day 14 of follow up reflects the role of semi-immunity protection and parasite clearance in the population. Conclusion In monitoring drug resistance to SP, concurrent studies on possible confounding factors pertaining to development of resistance in falciparum malaria should be considered. The SP resistance potential detected in this study, cautions on its useful therapeutic life as an interim first-line drug against malaria in Tanzania and other malaria-endemic countries. |
| وصف الملف: | application/octet-stream; application/pdf |
| تدمد: | 1475-2875 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ff19d272f4c8122e1d387a00ff1d624 https://pubmed.ncbi.nlm.nih.gov/17076899 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5ff19d272f4c8122e1d387a00ff1d624 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14752875 |
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