Characterization of the T-dependent antibody response (TDAR) to keyhole limpet hemocyanin (KLH) in the Göttingen minipig
| العنوان: | Characterization of the T-dependent antibody response (TDAR) to keyhole limpet hemocyanin (KLH) in the Göttingen minipig |
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| المؤلفون: | Donald G. Stump, Vanessa L. Peachee, Matthew J. Smith, Kimber L. White, Melissa J. Beck |
| المصدر: | Journal of Immunotoxicology. 11:376-382 |
| بيانات النشر: | Informa UK Limited, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Primates, Cyclophosphamide, Swine, Immunology, Immunization, Secondary, Miniature swine, chemical and pharmacologic phenomena, Toxicology, complex mixtures, Dogs, Antigen, medicine, Animals, biology, hemic and immune systems, T-Lymphocytes, Helper-Inducer, Göttingen minipig, Antibody response, Immunoglobulin M, Immunization, Immunoglobulin G, Antibody Formation, Hemocyanins, Models, Animal, biology.protein, Swine, Miniature, Immunocompetence, Immunosuppressive Agents, Keyhole limpet hemocyanin, medicine.drug |
| الوصف: | Recently, there has been a renewed interest in the use of the minipig as an alternative to dogs and non-human primates for conducting toxicological assessments in non-rodent species. Since the T-dependent antibody response (TDAR) is one of the most widely-accepted assays used in the assessment of immunocompetence, the present study was undertaken to characterize the primary and secondary TDAR to keyhole limpet hemocyanin (KLH) in the Göttingen Minipig(®). Following primary immunization with either 2 or 10 mg KLH, anti-swine IgM and IgG ELISAs were optimized and individual animal responses were evaluated over time. Immunization with 10 mg KLH on Day 0 promoted primary IgM responses that peaked 6-9 days after antigen administration, while primary IgG levels peaked on Day 13 or 14. Secondary IgG antibody levels (following secondary injection with 2 mg KLH on Day 14) plateaued on Days 20-22. Anti-KLH antibody levels were decreased in minipigs treated with cyclophosphamide (CPS), a known immunosuppressant, at doses ranging from 12.5-50 mg/kg/day, while antibody levels in animals treated with 2.5 mg CPS/kg/day were similar to levels in saline-treated swine. These results demonstrate that the Göttingen Minipig(®) can be a useful alternative non-rodent species to the dog and the non-human primate for evaluating the TDAR to KLH in regulatory assessments of immunotoxicity. |
| تدمد: | 1547-6901 1547-691X |
| DOI: | 10.3109/1547691x.2013.853716 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e186c4023557e58c72870bc19bdb29c https://doi.org/10.3109/1547691x.2013.853716 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5e186c4023557e58c72870bc19bdb29c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15476901 1547691X |
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| DOI: | 10.3109/1547691x.2013.853716 |