Metagenomics reveals sediment microbial community response to Deepwater Horizon oil spill
| العنوان: | Metagenomics reveals sediment microbial community response to Deepwater Horizon oil spill |
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| المؤلفون: | Nicole M. Scott, Olivia U. Mason, Julian L. Fortney, Antonio Gonzalez, Terry C. Hazen, Emmanuel Prestat, Janet K. Jansson, Diogo Jurelevicius, Nicholas J. Bouskill, William T. Stringfellow, Rob Knight, Sharon Borglin, Dominique C. Joyner, Jacob Bælum, Lisa Alvarez-Cohen, Jack A. Gilbert, Jeffrey A. Kimbrel, Adam Robbins-Pianka |
| المصدر: | The ISME journal, vol 8, iss 7 Mason, O U, Scott, N M, Gonzalez, A, Robbins-Pianka, A, Bælum, J, Kimbrel, J, Bouskill, N J, Prestat, E, Borglin, S, Joyner, D C, Fortney, J L, Jurelevicius, D, Stringfellow, W T, Alvarez-Cohen, L, Hazen, T C, Knight, R, Gilbert, J A & Jansson, J K 2014, ' Metagenomics reveals sediment microbial community response to Deepwater Horizon oil spill ', I S M E Journal, vol. 8, no. 7, pp. 1464-1475 . https://doi.org/10.1038/ismej.2013.254 The ISME Journal Mason, OU; Scott, NM; Gonzalez, A; Robbins-Pianka, A; Bælum, J; Kimbrel, J; et al.(2014). Metagenomics reveals sediment microbial community response to Deepwater Horizon oil spill. ISME Journal, 8(7), 1464-1475. doi: 10.1038/ismej.2013.254. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/3br5f2nd |
| بيانات النشر: | eScholarship, University of California, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | propylene 115-07-1 toxin, water pollutant, pollutant, Environmental Protection Agency, 22506, Toxicology - Environment and industry, 03502, Genetics - General, 10060, Biochemistry studies - General, Gammaproteobacterium 16S rRNA gene [Bacteria], Toxicology, Mixed Function Oxygenases, DEGRADING BACTERIA, RNA, Ribosomal, 16S, Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Facultatively Anaerobic Gram-Negative Rods [06700] Colwellia genus, Petroleum Pollution, Carbon Radioisotopes, hydrocarbons, Polycyclic Aromatic Hydrocarbons, genes, Gulf of Mexico, Ecology, aromatics, 37015, Public health - Air, water and soil pollution, Bacterial, water column, Population Studies, Polycyclic Hydrocarbons, Aromatic, Environmental biotechnology, iTag/Metagenomics, polycyclic aromatic hydrocarbons PAHs toxin, water pollutant, pollutant, GROWTH, deleterious impact, Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000] Gammaproteobacterium higher_taxa, Gammaproteobacteria, 16S, dodecane 112-40-3 toxin, water pollutant, pollutant, Environmental remediation, Nitrogen, Pollution Assessment Control and Management, ECOLOGY, Microbiology, Molecular Genetics, SDG 3 - Good Health and Well-being, Microbial ecology, Bacterial Proteins, Genetics, Seawater, nitrogen 7727-37-9, Ecology, Evolution, Behavior and Systematics, Ecosystem, Ribosomal, N762921K75 Nitrogen, metabolic process, Biochemistry and Molecular Biophysics, sequencing laboratory techniques, genetic techniques, SEQUENCES, Sediment, TAXONOMY, 15. Life on land, chemistry, Microbial population biology, Metagenomics, Colwellia 16S rRNA gene [Facultatively Anaerobic Gram-Negative Rods], Population Genetics, Technology, carbon-14 14762-75-5, DIVERSITY, Gene Expression, toluene 108-88-3 toxin, water pollutant, pollutant, deepwater horizon oil spill, chemistry.chemical_compound, METHANE, DWH oil spill, Geomicrobiology, Alteromonadaceae, sediments, 22501, Toxicology - General and methods, Biological Sciences, glycol 107-21-1 toxin, water pollutant, pollutant, RNA, Bacterial, microbial community structure, Original Article, microbial community, 03509, Genetics - Population genetics, GULF-OF-MEXICO, Biology, EC 1.- Mixed Function Oxygenases, deep-sea hydrocarbon plume, butane monooxygenase 9059-16-9 EC 1.14.15.3, 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines, phenanthrene 85-01-8 toxin, water pollutant, pollutant, 14. Life underwater, 31500, Genetics of bacteria and viruses, Phenanthrene, 31000, Physiology and biochemistry of bacteria, 13. Climate action, RNA, ecosystem remediation applied and field techniques, Environmental Sciences |
| الوصف: | The Deepwater Horizon (DWH) oil spill in the spring of 2010 resulted in an input of ∼4.1 million barrels of oil to the Gulf of Mexico; >22% of this oil is unaccounted for, with unknown environmental consequences. Here we investigated the impact of oil deposition on microbial communities in surface sediments collected at 64 sites by targeted sequencing of 16S rRNA genes, shotgun metagenomic sequencing of 14 of these samples and mineralization experiments using 14 C-labeled model substrates. The 16S rRNA gene data indicated that the most heavily oil-impacted sediments were enriched in an uncultured Gammaproteobacterium and a Colwellia species, both of which were highly similar to sequences in the DWH deep-sea hydrocarbon plume. The primary drivers in structuring the microbial community were nitrogen and hydrocarbons. Annotation of unassembled metagenomic data revealed the most abundant hydrocarbon degradation pathway encoded genes involved in degrading aliphatic and simple aromatics via butane monooxygenase. The activity of key hydrocarbon degradation pathways by sediment microbes was confirmed by determining the mineralization of 14 C-labeled model substrates in the following order: propylene glycol, dodecane, toluene and phenanthrene. Further, analysis of metagenomic sequence data revealed an increase in abundance of genes involved in denitrification pathways in samples that exceeded the Environmental Protection Agency (EPA)'s benchmarks for polycyclic aromatic hydrocarbons (PAHs) compared with those that did not. Importantly, these data demonstrate that the indigenous sediment microbiota contributed an important ecosystem service for remediation of oil in the Gulf. However, PAHs were more recalcitrant to degradation, and their persistence could have deleterious impacts on the sediment ecosystem. © 2014 International Society for Microbial Ecology All rights reserved. |
| وصف الملف: | application/pdf |
| DOI: | 10.1038/ismej.2013.254 |
| DOI: | 10.1038/ismej.2013.254. |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d440d525b0dfc747b8355fb0c88d312 https://escholarship.org/uc/item/3br5f2nd |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5d440d525b0dfc747b8355fb0c88d312 |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.1038/ismej.2013.254 |
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