Short-Term Test for Toxicogenomic Analysis of Ecotoxic Modes of Action in Lemna minor
| العنوان: | Short-Term Test for Toxicogenomic Analysis of Ecotoxic Modes of Action in Lemna minor |
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| المؤلفون: | Alexandra Loll, Hannes Reinwald, Steve U. Ayobahan, Bernd Göckener, Gabriela Salinas, Christoph Schäfers, Karsten Schlich, Gerd Hamscher, Sebastian Eilebrecht |
| المساهمون: | Publica |
| المصدر: | Environmental Science & Technology. 56:11504-11515 |
| بيانات النشر: | American Chemical Society (ACS), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | transcriptomics, HMG-CoA reductase inhibition, Lemna minor, proteomics, biomarkers, PSII inhibition, Environmental Chemistry, General Chemistry, functional annotation |
| الوصف: | In the environmental risk assessment of substances, toxicity to aquatic plants is evaluated using, among other methods, the 7 dayLemna sp. growth inhibition test following the OECD TG 221. So far, the test is not applicable for short-term screening of toxicity, nor does it allow evaluation of toxic modes of action (MoA). The latter is also complicated by the lack of knowledge of gene functions in the test species. Using ecotoxicogenomics, we developed a time-shortened 3 day assay inLemna minor which allows discrimination of ecotoxic MoA. By examining the changes in gene expression induced by low effect concentrations of the pharmaceutical atorvastatin and the herbicide bentazon at the transcriptome and proteome levels, we were able to identify candidate biomarkers for the respective MoA. We developed a homology-based functional annotation pipeline for the reference genome ofL. minor, which allowed overrepresentation analysis of the gene ontologies affected by both test compounds. Genes affected by atorvastatin mainly influenced lipid synthesis and metabolism, whereas the bentazon-responsive genes were mainly involved in light response. Our approach is therefore less time-consuming but sensitive and allows assessment of MoA in L. minor. Using this shortened assay, investigation of expression changes of the identified candidate biomarkers may allow the development of MoA-specific screening approaches in the future. |
| تدمد: | 1520-5851 0013-936X |
| DOI: | 10.1021/acs.est.2c01777 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d217129d03deaab84fe57b1a3f64493 https://doi.org/10.1021/acs.est.2c01777 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5d217129d03deaab84fe57b1a3f64493 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15205851 0013936X |
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| DOI: | 10.1021/acs.est.2c01777 |