Secretion and gene expression of secretory leukocyte protease inhibitor by human airway submucosal glands
| العنوان: | Secretion and gene expression of secretory leukocyte protease inhibitor by human airway submucosal glands |
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| المؤلفون: | T. Masuda, Kunio Shirato, Hiroki Saitoh, Sanae Shimura, Toshiaki Fushimi |
| المصدر: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 280:L79-L87 |
| بيانات النشر: | American Physiological Society, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Adult, Atropine, Male, Pulmonary and Respiratory Medicine, medicine.medical_specialty, Physiology, Proteinase Inhibitory Proteins, Secretory, Gene Expression, Bronchi, Muscarinic Antagonists, Respiratory Mucosa, Muscarinic Agonists, Biology, Dexamethasone, Exocrine Glands, Piperidines, Physiology (medical), Internal medicine, Gene expression, medicine, Humans, Secretory Leukocyte Peptidase Inhibitor, Secretion, RNA, Messenger, Glucocorticoids, Methacholine Chloride, Aged, Aged, 80 and over, Submucosal glands, Bronchus, Dose-Response Relationship, Drug, Proteins, Pirenzepine, Cell Biology, Middle Aged, Molecular biology, Protease inhibitor (biology), Trachea, medicine.anatomical_structure, Endocrinology, Neutrophil elastase, biology.protein, Female, Leukocyte Elastase, Respiratory tract, SLPI, medicine.drug |
| الوصف: | Submucosal glands were isolated within 4 h of death from tracheae and bronchi obtained from autopsied lungs, and the secretory response of secretory leukocyte protease inhibitor (SLPI) was examined with ELISA and a secretory index. Although human neutrophil elastase (HNE) at low concentrations increased SLPI secretion above the control level (i.e., 149% of control level at 10−11M), HNE at high concentrations significantly decreased it below the control level (i.e., 16% of control level at 10−7M). The decrease in SLPI concentration was shown to result from the degradation of SLPI by excessive HNE. Methacholine induced significant secretion (i.e., 363% of control level at 10−5M) that was abolished by both M1and M3receptor antagonists. A semiquantitative analysis of SLPI mRNA by RT-PCR and Southern blot showed that compared with the superficial epithelium, submucosal glands had a 30-fold or higher level of SLPI mRNA. Both HNE and methacholine significantly increased the level of SLPI mRNA in submucosal glands in a dose-dependent manner (i.e., 357% of control level at 10−7M and 175% of control level at 10−5M, respectively). These findings indicate that human airway submucosal glands can transcribe 30-fold or more SLPI mRNA than the superficial epithelium and that SLPI mRNA transcription and secretion are regulated by both HNE and muscarinic receptors. |
| تدمد: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.2001.280.1.l79 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b36ac8ce81432d54b310a6eb80bda7e https://doi.org/10.1152/ajplung.2001.280.1.l79 |
| رقم الانضمام: | edsair.doi.dedup.....5b36ac8ce81432d54b310a6eb80bda7e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221504 10400605 |
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| DOI: | 10.1152/ajplung.2001.280.1.l79 |