Oxidative Stress Modulates the Expression Pattern of Peroxiredoxin-6 in Peripheral Blood Mononuclear Cells of Asthmatic Patients and Bronchial Epithelial Cells
| العنوان: | Oxidative Stress Modulates the Expression Pattern of Peroxiredoxin-6 in Peripheral Blood Mononuclear Cells of Asthmatic Patients and Bronchial Epithelial Cells |
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| المؤلفون: | Jun Pyo Choi, So Young Park, Tae-Bum Kim, Woo-Jung Song, Sin Jeong Kim, Hyouk Soo Kwon, You Sook Cho, Keun Ai Moon, Hyun Jae Shim |
| المصدر: | Allergy, Asthma & Immunology Research |
| بيانات النشر: | The Korean Academy of Asthma, Allergy and Clinical Immunology and The Korean Academy of Pediatric Al, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Pulmonary and Respiratory Medicine, Immunology, hydrogen peroxide, Inflammation, peroxiredoxin-6, Cycloheximide, medicine.disease_cause, Peripheral blood mononuclear cell, Pathogenesis, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Immune system, post-translational modifications, medicine, oxidative stress, Immunology and Allergy, 030223 otorhinolaryngology, proteasome inhibitor, business.industry, respiratory system, Asthma, 030228 respiratory system, chemistry, airway, inflammation, Proteasome inhibitor, Original Article, medicine.symptom, business, Homeostasis, Oxidative stress, medicine.drug |
| الوصف: | Purpose Reduction-oxidation reaction homeostasis is vital for regulating inflammatory conditions and its dysregulation may affect the pathogenesis of chronic airway inflammatory diseases such as asthma. Peroxiredoxin-6, an important intracellular anti-oxidant molecule, is reported to be highly expressed in the airways and lungs. The aim of this study was to analyze the expression pattern of peroxiredoxin-6 in the peripheral blood mononuclear cells (PBMCs) of asthmatic patients and in bronchial epithelial cells (BECs). Methods The expression levels and modifications of peroxiredoxin-6 were evaluated in PBMCs from 22 asthmatic patients. Phosphorylated and acetylated peroxiredoxin-6 in hydrogen peroxide-treated human BECs was detected using immunoprecipitation analysis. The expression level of peroxiredoxin-6 was also investigated in BECs treated with hydrogen peroxide. Cycloheximide and proteasome inhibitors were used to determine whether peroxiredoxin-6 is degraded by proteasomes. Results Peroxiredoxin-6 expression was significantly reduced in the PBMCs of asthmatic patients compared to control subjects. Distinct modification patterns for peroxiredoxin-6 were observed in the PBMCs of asthmatic patients using 2-dimensional-electrophoresis. The levels of phosphorylated serine and acetylated lysine in peroxiredoxin-6 were significantly increased in the BECs following hydrogen peroxide treatment. The level of peroxiredoxin-6 expression was reduced in hydrogen peroxide-stimulated BECs, presumably due to proteasomes. Conclusions The expression of peroxiredoxin-6, which is down-regulated in the immune cells of asthmatic patients and BECs, can be modified by oxidative stress. This phenomenon may have an effect on asthmatic airway inflammation. |
| تدمد: | 2092-7363 2092-7355 |
| DOI: | 10.4168/aair.2020.12.3.523 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5acf87151fa10b91b8fe6426796c96cf https://doi.org/10.4168/aair.2020.12.3.523 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5acf87151fa10b91b8fe6426796c96cf |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20927363 20927355 |
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| DOI: | 10.4168/aair.2020.12.3.523 |