Computer-aided image analysis applied to immunogold-silver staining: evaluation of proliferating cell nuclear antigen (PCNA)-reactive sites in paraffin sections
| العنوان: | Computer-aided image analysis applied to immunogold-silver staining: evaluation of proliferating cell nuclear antigen (PCNA)-reactive sites in paraffin sections |
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| المؤلفون: | N. Utsumi, Yoshifumi Tajima, Kohtaro Kato, Kazuo Hosoi |
| المصدر: | Histochemistry. 102:177-181 |
| بيانات النشر: | Springer Science and Business Media LLC, 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | Silver Staining, Pathology, medicine.medical_specialty, Histology, medicine.drug_class, Cell, Monoclonal antibody, Antigen, Proliferating Cell Nuclear Antigen, Paraffin section, Immunogold silver staining, Image Processing, Computer-Assisted, medicine, Humans, Molecular Biology, Paraffin Embedding, biology, Cell Biology, General Medicine, Immunohistochemistry, Molecular biology, Staining, Proliferating cell nuclear antigen, Medical Laboratory Technology, medicine.anatomical_structure, biology.protein, Anatomy, General Agricultural and Biological Sciences, Cytometry |
| الوصف: | Feasibility of the combination of the immunogold-silver staining method (IGSS) and computer-aided image analysis was assessed for the detection of antigen in an immunostained, paraffin-embedded section. Using low-temperature IGSS, we stained a specimen of human oral squamous cell carcinoma with a monoclonal antibody, PC 10, against a proliferating cell nuclear antigen (PCNA/cyclin), and the section was analyzed by ACAS 570 interactive laser cytometry. The PCNA-positive cells, exhibiting a heteromorphic texture, were contrasted by the dark staining of their nuclei, but showed heterogeneity in staining intensity from cell to cell. Using a conventional microscope light source rather than a laser, and by employing the COMPLEMENT DATA program (which permits inversion of the data values) installed in the ACAS 570 software system, we were able to obtain a 'complemental image' which replicated the real immunohisto-morphology. Approximately 30-35 cells from three different areas in the same section were selected by DEFINE CELL and MARK AREA programs, and quantitative image analysis was performed in terms of cell integrated value, area, perimeter, and shape factor indicated in histogram form. The combined utilization of IGSS with computer-aided image analysis was demonstrated to offer a crucial advantage for the quantitative assessment of immunostained sections. |
| تدمد: | 1432-119X 0301-5564 |
| DOI: | 10.1007/bf00268894 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::593a80322d0b56befd394693bbe06142 https://doi.org/10.1007/bf00268894 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....593a80322d0b56befd394693bbe06142 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1432119X 03015564 |
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| DOI: | 10.1007/bf00268894 |