Cancer stem cells from human glioblastoma resemble but do not mimic original tumors after in vitro passaging in serum-free media
| العنوان: | Cancer stem cells from human glioblastoma resemble but do not mimic original tumors after in vitro passaging in serum-free media |
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| المؤلفون: | Maria Peris-Celda, Carmen González-Tejedo, Cristobal Belda-Iniesta, Susana Esteban-Rubio, David Blesa, Rosario Perona, Vanessa Rodríguez-Fanjul, Laura Ramírez-Jiménez, Noemí García-Romero, Angel Ayuso-Sacido, Carmen Escobedo-Lucea, Gorjana Rackov, Ricardo Prat-Acín, Jorge Oliver-De La Cruz, Josefa Carrión-Navarro, Pilar Sánchez-Gómez |
| المساهمون: | Ministerio de Economía y Competitividad (España), Instituto de Salud Carlos III, Red Temática de Investigación Cooperativa en Cáncer (España), European Commission, European Regional Development Fund, Division of Pharmaceutical Biosciences, Faculty of Pharmacy |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname Oncotarget r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF) Centro de Investigación Principe Felipe (CIPF) Repisalud Instituto de Salud Carlos III (ISCIII) r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF) Repositorio Institucional del Instituto Madrileño de Estudios Avanzados en Nanociencia |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, cancer stem cells, Cell Culture Techniques, ComputingMilieux_LEGALASPECTSOFCOMPUTING, Apoptosis, PHENOTYPE, Stem cell marker, Culture Media, Serum-Free, Metastasis, Mice, Tumor Cells, Cultured, EPIDEMIOLOGY, HETEROGENEITY, PRECURSORS, Mice, Inbred BALB C, Brain Neoplasms, Drug discovery, Cancer stem cells, 1. No poverty, TEMOZOLOMIDE, Prognosis, GENOTYPE, 3. Good health, Oncology, 317 Pharmacy, Neoplastic Stem Cells, GLIOMA, Female, medicine.drug, Research Paper, 3122 Cancers, Mice, Nude, Context (language use), Antineoplastic Agents, Biology, In Vitro Techniques, drug discovery, 03 medical and health sciences, Cancer stem cell, Glioma, medicine, Biomarkers, Tumor, Animals, Humans, Primary cell culture, Cell Proliferation, Temozolomide, genetic alterations, IDENTIFICATION, CENTRAL-NERVOUS-SYSTEM, glioblastoma, PROFILES, medicine.disease, Xenograft Model Antitumor Assays, In vitro, 030104 developmental biology, Cell culture, Cancer research, Glioblastoma, Genetic alterations, primary cell culture |
| الوصف: | This is an open-access article distributed under the terms of the Creative Commons Attribution License.-- et al. Human gliomas harbour cancer stem cells (CSCs) that evolve along the course of the disease, forming highly heterogeneous subpopulations within the tumour mass. These cells possess self-renewal properties and appear to contribute to tumour initiation, metastasis and resistance to therapy. CSC cultures isolated from surgical samples are considered the best preclinical in vitro model for primary human gliomas. However, it is not yet well characterized to which extent their biological and functional properties change during in vitro passaging in the serum-free culture conditions. Here, we demonstrate that our CSC-enriched cultures harboured from one to several CSC clones from the human glioma sample. When xenotransplanted into mouse brain, these cells generated tumours that reproduced at least three different dissemination patterns found in original tumours. Along the passages in culture, CSCs displayed increased expression of stem cell markers, different ratios of chromosomal instability events, and a varied response to drug treatment. Our findings highlight the need for better characterization of CSC-enriched cultures in the context of their evolution in vitro, in order to uncover their full potential as preclinical models in the studies aimed at identifying molecular biomarkers and developing new therapeutic approaches of human gliomas. We are grateful for the financial support from PI10/01069, PI14/00077, and the ‘Miguel Servet Program’ CP11/00147 (AAS), PI-01495 (RP), and PI12/00775) (PSG) from Fondo de Investigaciones Sanitarias, Instituto de Salud Carlos III, Spain, supported by FEDER funds, and Ministerio de Economía y Competitividad, Red Temática de Investigación Cooperativa en Cáncer (RTICC) (RD12/0036/0027) (PSG). |
| اللغة: | English |
| تدمد: | 1949-2553 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::586a3bff43f137221f40940bcea92f8e http://hdl.handle.net/20.500.12614/2436 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....586a3bff43f137221f40940bcea92f8e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19492553 |
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