Extracellular-Matrix-Reinforced Bioinks for 3D Bioprinting Human Tissue
| العنوان: | Extracellular-Matrix-Reinforced Bioinks for 3D Bioprinting Human Tissue |
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| المؤلفون: | Jeffery A. Wood, Chiharu Ota, Manlio Tassieri, Martina M. De Santis, John Stegmayr, Margareta Mittendorfer, Sofie Mohlin, Paul Bourgine, Deniz A. Bölükbas, Iran A. Silva, Sandra Lindstedt, Darcy E. Wagner, Hani N. Alsafadi, Melanie Königshoff, Sinem Tas, Sujeethkumar Prithiviraj, Karl Swärd, Fatima Daoud |
| المساهمون: | MESA+ Institute, Soft matter, Fluidics and Interfaces |
| المصدر: | Advanced Materials Adv. Mater. 33:2005476 (2021) Advanced materials, 33(3):2005476. Wiley-Blackwell |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Materials science, bioinks, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, law.invention, Extracellular matrix, 3d Bioprinting, Biofabrication, Bioinks, Extracellular Matrix, Tissue Engineering, Mice, Tissue engineering, law, medicine, Animals, Humans, General Materials Science, 3D bioprinting, Decellularization, Tissue Scaffolds, Mechanical Engineering, biofabrication, Bioprinting, 021001 nanoscience & nanotechnology, Epithelium, 0104 chemical sciences, Cell biology, medicine.anatomical_structure, Mechanics of Materials, tissue engineering, Self-healing hydrogels, Printing, Three-Dimensional, Ink, 0210 nano-technology, Ex vivo |
| الوصف: | Recent advances in 3D bioprinting allow for generating intricate structures with dimensions relevant for human tissue, but suitable bioinks for producing translationally relevant tissue with complex geometries remain unidentified. Here, a tissue-specific hybrid bioink is described, composed of a natural polymer, alginate, reinforced with extracellular matrix derived from decellularized tissue (rECM). rECM has rheological and gelation properties beneficial for 3D bioprinting while retaining biologically inductive properties supporting tissue maturation ex vivo and in vivo. These bioinks are shear thinning, resist cell sedimentation, improve viability of multiple cell types, and enhance mechanical stability in hydrogels derived from them. 3D printed constructs generated from rECM bioinks suppress the foreign body response, are pro-angiogenic and support recipient-derived de novo blood vessel formation across the entire graft thickness in a murine model of transplant immunosuppression. Their proof-of-principle for generating human tissue is demonstrated by 3D bioprinting human airways composed of regionally specified primary human airway epithelial progenitor and smooth muscle cells. Airway lumens remained patent with viable cells for one month in vitro with evidence of differentiation into mature epithelial cell types found in native human airways. rECM bioinks are a promising new approach for generating functional human tissue using 3D bioprinting. |
| وصف الملف: | application/pdf |
| تدمد: | 1521-4095 0935-9648 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::584ae57c7eace0307842eb18d8916cb9 https://pubmed.ncbi.nlm.nih.gov/33300242 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....584ae57c7eace0307842eb18d8916cb9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15214095 09359648 |
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