Intestinal microbiome adjusts the innate immune setpoint during colonization through negative regulation of MyD88
| العنوان: | Intestinal microbiome adjusts the innate immune setpoint during colonization through negative regulation of MyD88 |
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| المؤلفون: | Bjørn E. V. Koch, Shuxing Yang, Herman P. Spaink, Gerda E. M. Lamers, Jens Stougaard |
| المصدر: | Nature Communications Nature Communications, 9, 4099 Nature Communications, Vol 9, Iss 1, Pp 1-11 (2018) Koch, B E V, Yang, S, Lamers, G, Stougaard, J & Spaink, H P 2018, ' Intestinal microbiome adjusts the innate immune setpoint during colonization through negative regulation of MyD88 ', Nature Communications, vol. 9, no. 1, 4099 . https://doi.org/10.1038/s41467-018-06658-4 |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, animal structures, Science, General Physics and Astronomy, Article, General Biochemistry, Genetics and Molecular Biology, Microbiology, 03 medical and health sciences, 0302 clinical medicine, Immune system, Immunity, Gene expression, Colonization, Microbiome, lcsh:Science, Zebrafish, Multidisciplinary, Innate immune system, biology, fungi, General Chemistry, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, TLR2, 030104 developmental biology, 030220 oncology & carcinogenesis, lcsh:Q |
| الوصف: | Host pathways mediating changes in immune states elicited by intestinal microbial colonization are incompletely characterized. Here we describe alterations of the host immune state induced by colonization of germ-free zebrafish larvae with an intestinal microbial community or single bacterial species. We show that microbiota-induced changes in intestinal leukocyte subsets and whole-body host gene expression are dependent on the innate immune adaptor gene myd88. Similar patterns of gene expression are elicited by colonization with conventional microbiome, as well as mono-colonization with two different zebrafish commensal bacterial strains. By studying loss-of-function myd88 mutants, we find that colonization suppresses Myd88 at the mRNA level. Tlr2 is essential for microbiota-induced effects on myd88 transcription and intestinal immune cell composition. It remains unclear how microbial sensing during early-life colonization results in immune homeostasis rather than acute inflammation. Here the authors show that zebrafish larvae colonization suppresses intestinal MyD88, accounting for a considerable proportion of microbiota-induced alterations in immune setpoint. |
| وصف الملف: | application/pdf |
| تدمد: | 2041-1723 |
| DOI: | 10.1038/s41467-018-06658-4 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57d1acd1a260c67dbe890244da60b4d0 https://doi.org/10.1038/s41467-018-06658-4 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....57d1acd1a260c67dbe890244da60b4d0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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| DOI: | 10.1038/s41467-018-06658-4 |