Examination of genetic diversity in common bean (Phaseolus vulgaris L.) using random amplified polymorphic DNA (RAPD) markers

التفاصيل البيبلوغرافية
العنوان: Examination of genetic diversity in common bean (Phaseolus vulgaris L.) using random amplified polymorphic DNA (RAPD) markers
المؤلفون: Asifa Bukhari, Noorul Saleem, Mashooq A. Bhat, Mushtaq Ahmad
المصدر: African Journal of Biotechnology; Vol 14, No 6 (2015); 451-458
بيانات النشر: Academic Journals, 2015.
سنة النشر: 2015
مصطلحات موضوعية: Genetics, Veterinary medicine, Genetic diversity, Genetic diversity, polymorphism, cluster analysis, random amplified polymorphic DNA markers (RAPD), common bean, Biology, biology.organism_classification, Applied Microbiology and Biotechnology, RAPD, Similarity (network science), Genetic marker, Polymorphism (computer science), Genotype, Primer (molecular biology), Phaseolus, Agronomy and Crop Science, Molecular Biology, Biotechnology
الوصف: To study the pattern of genetic diversity in 45 genotypes of common bean, 19 RAPD primers were used. Of 253 bands produced, 236 bands (94.22%) were polymorphic in which maximum number (20 polymorphic bands) were observed in the profiles of the primer OPB-07. Highest PIC value (0.79) was observed for the primers OPG-14 and OPE-1, whereas lowest PIC value (0.34) was recorded for the primer OPA-11 with an average PIC value of 0.54. Similarly, highest effective multiplex ratio value (0.11) was scored for the primers OPA-04 and OPG-6 and the lowest value (0.05) was recorded for the primer OPB-07. Primer OPG-14 and OPE-1 exhibited the highest marker index value (0.078) and the lowest value (0.024) was recorded for OPA-11. Pair-wise genetic similarity coefficients of genotypes varied from 0.56 to 0.92 with an average of 0.70 amongst 45 genotypes. The maximum similarity coefficient (0.92) was noticed between G-9 and G-8. Cluster analysis separated 45 genotypes into seven major clusters which were further grouped into various sub-clusters. Among these seven clusters, the maximum number of genotypes (12) were recorded in cluster I, while cluster VII formed a mono-genotypic cluster. Key words: Genetic diversity, polymorphism, cluster analysis, random amplified polymorphic DNA markers (RAPD), common bean.
وصف الملف: application/pdf
تدمد: 1684-5315
DOI: 10.5897/ajb2014.14281
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57af4d128d099118e7bf4eae418dc7bd
https://doi.org/10.5897/ajb2014.14281
Rights: OPEN
رقم الانضمام: edsair.doi.dedup.....57af4d128d099118e7bf4eae418dc7bd
قاعدة البيانات: OpenAIRE
الوصف
تدمد:16845315
DOI:10.5897/ajb2014.14281