Impact of the Yosemite Hantavirus Outbreak on Hantavirus Antibody Testing at a National Reference Laboratory
| العنوان: | Impact of the Yosemite Hantavirus Outbreak on Hantavirus Antibody Testing at a National Reference Laboratory |
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| المؤلفون: | Harry E. Prince, Jay M. Lieberman |
| المصدر: | Clinical and Vaccine Immunology. 20:1213-1216 |
| بيانات النشر: | American Society for Microbiology, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Veterinary Medicine, Microbiology (medical), Sin Nombre virus, Hantavirus Infections, viruses, Clinical Biochemistry, Immunology, Antibodies, Viral, Immunoglobulin G, Disease Outbreaks, Diagnostic Laboratory Immunology, medicine, Humans, Immunology and Allergy, Hantavirus, Immunoassay, medicine.diagnostic_test, biology, Clinical Laboratory Techniques, business.industry, Outbreak, Virology, United States, Immunoglobulin M, biology.protein, Antibody, Hantavirus Infection, business, Algorithms |
| الوصف: | In conjunction with the 2012 Yosemite hantavirus outbreak, the number of sera our facility tested for hantavirus antibodies increased. We tracked test results and used the data set to determine if a more efficient testing algorithm was possible. Sera were screened using laboratory-developed pan-hantavirus IgG and IgM enzyme immunoassays (EIAs), with an index of >1.10 defined as positive. Sera that were IgM positive by screening (screen IgM + ) were tested for Sin Nombre virus (SNV)-specific IgM using a laboratory-developed EIA; screen IgM + IgG + sera were also tested for SNV IgG using a laboratory-developed immunoblot assay. SNV antibody-positive samples were sent to state public health laboratories (PHL) or the CDC for confirmation. Of 3,946 sera tested from July through December 2012, 205 were screen IgM + IgG negative (IgG − ); 7/205 were SNV IgM + , but only 1/5 sent to PHL/CDC was confirmed as SNV IgM + . Of 61 screen IgM + IgG + sera, 16 were SNV antibody positive; 13/16 sera (from 11 patients) went to PHL/CDC, where SNV infection was confirmed for all patients. Of 12 confirmed patients, 7 had been exposed at Yosemite. A modified algorithm defining screen indices of ≥2.00 as positive identified 11/12 confirmed cases while reducing the number of sera requiring SNV-specific antibody testing by 65%; the patient missed was not tested until 3 months after the onset of symptoms. Hantavirus antibody testing at our facility identified 12 SNV-infected patients, including 7 exposed at Yosemite. Some screen IgM + IgG − SNV IgM + results were false positives, emphasizing the value of PHL/CDC confirmatory testing. We identified a modified algorithm requiring analysis of fewer specimens for SNV-specific antibodies without loss of sensitivity. |
| تدمد: | 1556-679X 1556-6811 |
| DOI: | 10.1128/cvi.00326-13 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5788626fceaed3e6951e9c4188098e5a https://doi.org/10.1128/cvi.00326-13 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5788626fceaed3e6951e9c4188098e5a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1556679X 15566811 |
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| DOI: | 10.1128/cvi.00326-13 |