Allergic sensitization increases the amount of extracellular ATP hydrolyzed by guinea pig leukocytes
| العنوان: | Allergic sensitization increases the amount of extracellular ATP hydrolyzed by guinea pig leukocytes |
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| المؤلفون: | Jaime Chávez, Ramcés Falfán-Valencia, Mario H. Vargas, Verónica Carbajal, Jesús Martínez-Zúñiga, Rosa Sandoval-Roldán, Enrique Ambrocio-Ortiz |
| المصدر: | Purinergic Signalling. 15:69-76 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, medicine.medical_specialty, Guinea Pigs, Stimulation, Allergic sensitization, 03 medical and health sciences, Cellular and Molecular Neuroscience, Adenosine Triphosphate, 0302 clinical medicine, ATP hydrolysis, Internal medicine, Hypersensitivity, Leukocytes, medicine, Extracellular, Animals, Ectonucleotidase, Molecular Biology, Sensitization, Receptors, Purinergic P2, Chemistry, Hydrolysis, Purinergic receptor, Cell Biology, Adenosine, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, Original Article, Receptors, Purinergic P2X7, 030217 neurology & neurosurgery, medicine.drug |
| الوصف: | Increased levels of ATP have been found in the bronchoalveolar lavage of patients with asthma, and subjects with this disease, but not healthy subjects, develop bronchospasm after nebulization with ATP. Because the main mechanism for controlling the noxious effects of extracellular ATP is its enzymatic hydrolysis, we hypothesized that allergic sensitization is accompanied by a decreased functioning of such hydrolysis. In the present study, peripheral blood leukocytes from sensitized and non-sensitized guinea pigs were used for determining the extracellular metabolism (as assessed by inorganic phosphate production) of ATP, ADP, AMP, or adenosine, and for detecting possible changes in the expression (qPCR and Western blot) of major ectonucleotidases (NTPDase1, NTPDase3, and NPP1) and purinoceptors (P2X(1), P2X(7), P2Y(4), and P2Y(6)). Contrary to our hypothesis, we found that leukocytes from allergic animals produced higher amounts of inorganic phosphate after stimulation with ATP and ADP, as compared with leukocytes from non-sensitized animals. Although at first glance, this result suggested that sensitization caused higher efficiency of ectonucleotidases, their mRNA and protein expressions were unaffected. On the other hand, after sensitization, we found a significant increase in the protein expression of P2X(7) and P2Y(4), two purinoceptors known to be responsible for ATP release after activation. We concluded that allergic sensitization increased the amount of ATP hydrolyzed by ectonucleotidases, the latter probably not due to the enhanced efficiency of its enzymatic breakdown, but rather due to an increased release of endogenous ATP or other nucleotides, partly mediated by enhanced expression or P2X(7) and P2Y(4) receptors. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11302-019-09644-7) contains supplementary material, which is available to authorized users. |
| تدمد: | 1573-9546 1573-9538 |
| DOI: | 10.1007/s11302-019-09644-7 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57534c49688415148702ebf3d7d71b19 https://doi.org/10.1007/s11302-019-09644-7 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....57534c49688415148702ebf3d7d71b19 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15739546 15739538 |
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| DOI: | 10.1007/s11302-019-09644-7 |