Deep Paleoproteotyping and Microtomography Revealed No Heart Defect nor Traces of Embalming in the Cardiac Relics of Blessed Pauline Jaricot
| العنوان: | Deep Paleoproteotyping and Microtomography Revealed No Heart Defect nor Traces of Embalming in the Cardiac Relics of Blessed Pauline Jaricot |
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| المؤلفون: | Virginie Bourdin, Philippe Charlier, Stéphane Crevat, Lotfi Slimani, Catherine Chaussain, Mélodie Kielbasa, Olivier Pible, Jean Armengaud |
| المساهمون: | Musée du quai Branly – Jacques Chirac (MQBJC), Laboratoire Anthropologie, Archéologie, Biologie (LAAB), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay, Pathologies, imagerie et biothérapies oro-faciales = Orofacial pathologies, imaging and biotherapies (URP 2496), Université Paris Cité (UPCité), Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Région Occitanie, (21023526-DeepMicro, ANR-11- INBS-0006, FRM DGE20111123012), Agence Nationale de la Recherche, ANR, (ANR-20-CE34-0012), ANR-20-CE34-0012,Dyn-Microbiome,Dynamique du microbiome d'un organisme sentinelle aquatique en fonction de contaminants(2020), ANR-11-INBS-0006,FLI,France Life Imaging(2011) |
| المصدر: | International Journal of Molecular Sciences International Journal of Molecular Sciences, 2023, 24 (3), pp.3011. ⟨10.3390/ijms24033011⟩ Volume 24 Issue 3 Pages: 3011 |
| بيانات النشر: | HAL CCSD, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | paleopathology, forensic anthropology, Organic Chemistry, micro-CT scan, [SHS.ANTHRO-BIO]Humanities and Social Sciences/Biological anthropology, proteotyping, relics, General Medicine, Catalysis, Computer Science Applications, Inorganic Chemistry, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], metaproteomics, cardiac characterization, Physical and Theoretical Chemistry, Molecular Biology, Spectroscopy, mass spectrometry |
| الوصف: | International audience; Scientific examination of the heart of Blessed Pauline Jaricot—a French missionary figure—was carried out in 2022. As tandem mass spectrometry proteotyping has proven to be valuable to obtain the broad taxonomic repertoire of a given sample without any a priori information, we aimed at exploring the conditions of preservation of the relics and possible conditions of death. Metaproteomics and high-resolution microtomography imaging approaches were combined. A dataset comprising 6731 high-resolution MS/MS spectra was acquired and 968 of these spectra could be assigned to specific peptidic biomolecules. Based on the taxonomical information encompassed by the identified peptide sequences, 5 phyla were identified amongst eukaryota (94% of the biomass): Ascomycota (55%), with the species Aspergillus versicolor, Trichophyton mentagrophytes and Aspergillus glaucus, corresponding to expected cadaverous fungal flora; Chordata (42%), represented by a unique species, Homo sapiens; Streptophyta (3%); and Arthropoda (traces). Bacteria (6% of the biomass) were poorly represented. No trace of embalming substance could be retrieved, nor any pathogens. Imaging evidenced no heart defect nor embalming traces. No evidence that was inconsistent with natural and spontaneous conservation could be retrieved. This study prefigures the power of modern molecular techniques such as paleoproteotyping coupled to microtomography to gain insight into historical relics. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1661-6596 1422-0067 |
| DOI: | 10.3390/ijms24033011⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56fb13b7218629606586b67bddb78cb2 https://hal.science/hal-04069285 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....56fb13b7218629606586b67bddb78cb2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16616596 14220067 |
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| DOI: | 10.3390/ijms24033011⟩ |