Cytogenetic and molecular identification of small-segment chromosome translocation lines from wheat-rye substitution lines to create wheat germplasm with beneficial traits
| العنوان: | Cytogenetic and molecular identification of small-segment chromosome translocation lines from wheat-rye substitution lines to create wheat germplasm with beneficial traits |
|---|---|
| المؤلفون: | Wen-Li Xin, Wei-Fu Song, Chun-Li Zhang, Qing-jie Song, Yan-Bin Zhang, Hai-Yan Ding, Zhi-Min Xiao, Ji-Lin Li, Hai-Bin Zhao, Xiao-Mei Zhang |
| المصدر: | Biotechnology, Biotechnological Equipment |
| بيانات النشر: | Informa UK Limited, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Genetics, Germplasm, substitution line hybridization, food and beverages, genomic in situ hybridization, Chromosomal translocation, Biology, Plant disease resistance, C-banding, Homology (biology), Chromatin, wheat variety trials, Meiosis, Articles, Agriculture & Environmental Biotechnology, GenBank, Gene, Biotechnology |
| الوصف: | Intergeneric crop plant hybrid lines with small-segment chromosome translocations are very useful in plant genetic research and breeding. In this study, to create small-segment chromosome translocations with beneficial agronomic characters, the progeny of wheat-rye substitution lines 5R/5A and 6R/6A were selected from generations F2 to F5 for rye-specific characteristics. A PCR primer and specific simple sequence repeat marker for rye were used in F5 populations to detect rye chromatin and to amplify a specific chromosome band in six translocation lines (06-6-5, 06-6-6, 06-6-9, 6-26-1, 7-23, and 7-33). Fragment pSc119.1 cloned from 7-33 had 99% homology with the big ear gene sequence (GenBank AF512607.1) in wheat. The six lines were further characterized via pollen mother cell meiosis analysis for genetic stability, and chromosome C-banding and genomic in situ hybridization for rye chromatin. The results show that line 7-33 was still within the 5R/5A substitution lines and possessed the big ear gene. The other lines all contained small-segment rye chromosome translocations. The results indicated that substitution line hybridization is an effective method for creating small-segment chromosome translocations with useful agronomic traits. Trials for these six wheat-rye translocation lines are justified because they possess many important stably-inherited agronomic characters, including disease resistance and improved yield. |
| تدمد: | 1314-3530 1310-2818 |
| DOI: | 10.1080/13102818.2014.901689 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55fface6af3fb3cb4f554b908a787b69 https://doi.org/10.1080/13102818.2014.901689 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....55fface6af3fb3cb4f554b908a787b69 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13143530 13102818 |
|---|---|
| DOI: | 10.1080/13102818.2014.901689 |